Synonyms: 9-(2-Carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride

Description

Rhodamine B is a classic xanthene‑based fluorescent dye (molecular formula C₈H₃₁ClN₂O₃, molecular weight ~479.02 g/mol) widely used in biological, environmental, and chemical research. This reagent‑grade powder from BioFargo contains approximately 80% dye content, offering strong fluorescence and versatile solubility. It dissolves readily in water, ethanol, or methyl cellosolve and is slightly soluble in acetone, making it convenient for preparing staining solutions, tracer studies, or dye‑laser media under various lab conditions. The dye exhibits a characteristic absorption maximum around 543–545 nm (in methanol), consistent with literature values.

BioFargo’s Rhodamine B is supplied as a green powder — the typical appearance for raw Rhodamine B salts — and is provided with Certificate of Analysis (COA) and Safety Data Sheet (SDS) for research‑grade applications. It is intended strictly for laboratory research use.

Specifications

Item	Value
Chemical Name	Rhodamine B
Appearance	Green Powder
CAS No.	81-88-9
Molecular Formula	C28H31ClN2O3
Molecular Weight	479.02
Grade	Reagent Grade
Dye Content	~80%
Lambda Max	543(355)nm in Methanol
Solubility	Soluble in Water, Ethanol and Methyl Cellosolve and Slightly Soluble in Acetone.
Storage	Store at Room Temperature.

Features

Reagent‑grade quality with ~80% dye content — suitable for research, staining, and tracer applications.
Well‑defined chemical specs — CAS 81‑88‑9, molecular formula C₂₈H₃₁ClN₂O₃, molecular weight ≈ 479.02 g/mol.
Strong fluorescence — absorption maxima around 543–545 nm; efficient excitation/emission makes it useful in microscopy, fluorescence assays, and dye‑laser media.
Good solubility in common solvents — water, ethanol, and methyl cellosolve; slight solubility in acetone; can be readily dissolved for preparing stock solutions.
Versatile utility — applicable for fluorescence staining, environmental tracing, tracer studies in water flow/dispersion, dye‑laser research, and other lab applications.

Applications

Fluorescence microscopy, histology, or cytology — e.g., as a fluorescent stain for cells, tissues, or microbial samples.
Flow cytometry and fluorescence‑based assays (e.g., ELISA, fluorescence correlation spectroscopy) for biomolecule tracking and quantification.
Environmental and hydrological studies — as a tracer dye to monitor water flow, dispersion, pollutant tracing, or mixing behavior in surface water, groundwater, or waste water.
Dye‑laser research and photophysical studies — leveraging its xanthene‑based fluorescent properties for laser dyes, photophysics, or optical experiments.
General chemical research — preparing fluorescent solutions, labeling experiments, solvent & stability studies, or developing custom fluorescent probes.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

COA

SDS

Rhodamine B 80% Reagent Grade