{"product_id":"rfp-expressing-monkey-primary-colonic-microvascular-endothelial-cells","title":"RFP Expressing Monkey Primary Colonic Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRFP-Expressing Monkey Primary Colonic Microvascular Endothelial Cells from Cell Biologics are isolated from Cynomolgus Monkey colon tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. RFP-Expressing Monkey Primary Colonic Microvascular Endothelial are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. ab9498, Abcam), VE-cadherin (Catalog No. sc-28644, Santa Cruz, or Catalog No. ALX-210-232-C100, Enzo Life Sciences, Inc.), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\n\u003cp\u003eRed Fluorescent Protein (RFP), a mutant form of green fluorescent protein, gives rise to very bright red fluorescence when exposed to yellow-green light. Cell Biologics generates various RFP-expressing stable cells, including endothelial cells, epithelial cells and so forth. RFP-expressing cells are developed through transducing RFP-lentiviral particles.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eMK1168 Complete Monkey Endothelial Cell Medium – 125 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/MK-6203RFP_MK-6203RFP.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMK-6203RFP\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/MK-6203RFP_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/MK-6203RFP_1708462493.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003e1708462493\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/MK-6203RFP_1698246847.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003e1698246847\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at\u003c\/p\u003e\n\u003cp\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":52481697579189,"sku":"MK-6203RFP","price":1100.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/no_image-500x500_b61d5696-530f-48bb-92b9-999e4a574928.webp?v=1778264924","url":"https:\/\/biofargo.com\/products\/rfp-expressing-monkey-primary-colonic-microvascular-endothelial-cells","provider":"Biofargo","version":"1.0","type":"link"}