Description

RFP-Expressing Mouse Primary Esophageal Epithelial Cells from Cell Biologics are isolated from the esophageal tissues of pathogen-free laboratory C57BL/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. RFP-Expressing Mouse Primary Esophageal Epithelial Cells are characterized by immunofluorescent staining with E-cadherin (Catalog 610182, BD) or ZO-1 (Catalog 617300, Life Technology) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

Red Fluorescent Protein (RFP), a mutant form of green fluorescent protein, gives rise to very bright red fluorescence when exposed to yellow-green light. Cell Biologics generates various RFP-expressing stable cells, including endothelial cells, epithelial cells and so forth. RFP-expressing cells are developed through transducing RFP-lentiviral particles.

Suggested Medium

M6621 Complete Epithelial Cell Medium w/ Kit – 500 ml

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

C57-6046RFP

Cell-Culture-Protocol-CB

1708462493

1698246847

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

RFP Expressing C57BL/6 Mouse Primary Esophageal Epithelial Cells