{"product_id":"revertra-ace-qpcr-rt-master-mix-gdna-remover","title":"ReverTra Ace qPCR RT Master Mix gDNA Remover-Toyobo","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eThis kit includes reagents for reverse transcription and for the removal of genomic DNA [DNase I treatment].\u003c\/p\u003e\n\u003cp\u003eIn many cases, total RNA prepared using spin-columns or acid guanidium-phenolchloroform (AGPC) extraction methods contains small amount of genomic DNA. Any contaminating genomic DNA will be amplified along with cDNA, especially when primer pairs are designed within the same exon or from pseudogenes. Amplification from genomic DNA can result in qualitative and quantitative inaccuracies. The protocol consists of (i) a genomic DNA degradation step using \"gDNA remover\" and (ii) a reverse transcription step. The two steps can be achieved sequentially without purification or heat inactivation of DNase I.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding-left: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \"Genomic DNA degradation step\" and \"cDNA synthesis step\" can be achieved sequentially in approximately 30 min.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e The master mix reagent contains random and oligo dT primers optimized for efficient reverse transcription.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e Control, no reverse transcription experiments (no RT-control) can be performed with 5x RT Master Mix II no-RT control.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n  \u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic01_b0d629d1-d7bf-42b2-a5f4-6f727610b947.png?v=1775789296\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eItem\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc;\"\u003eStorage condition\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eStore at -20ºC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"5\" style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003egDNA Remover\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e10 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e4x DN Master Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e440 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e5x RT Master Mix II\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e400 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e5x RT Master Mix II no RT-Control\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e40 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eNuclease-free water\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1000 µL × 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication\u003c\/h2\u003e\n\u003cp style=\"padding-left: 5px;\"\u003ecDNA synthesis for real-time PCR\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication Data\u003c\/h2\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample.Efficiency of genomic DNA removal\u003c\/h3\u003e\n\u003cp\u003eIn experiment 1, reverse transcription was performed according to the following condition and Table 1. Then β-actin genes were detected by SYBR® Green I assay. No signal for the \"C experiment\" indicates that contaminating genomic DNA in the RNA template was completely removed by \"gDNA remover\".\u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto; margin: 20px 0;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 500px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e\u003c\/th\u003e\n\u003cth style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e4x DN Master Mix\u003c\/th\u003e\n\u003cth style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e5x RT Master Mix\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; font-weight: bold;\"\u003eA\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003egDNA Remover(-)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eRTase(-)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; font-weight: bold;\"\u003eB\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003egDNA Remover(-)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eRTase(+)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; font-weight: bold;\"\u003eC\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003egDNA Remover(+)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eRTase(-)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; font-weight: bold;\"\u003eD\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003egDNA Remover(+)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eRTase(+)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic02_b395c9d8-7ca0-44e6-82f1-7b36b29b3fa2.png?v=1775204553\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003cp\u003eIn experiment 1, reverse transcription was performed according to the following condition and Table 1. Then β-actin genes were detected by SYBR® Green I assay. No signal for the \"C experiment\" indicates that contaminating genomic DNA in the RNA template was completely removed by \"gDNA remover\".\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic03.png?v=1775204553\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eTemplate RNA\u003c\/strong\u003e\u003cbr\u003eExperiment 1: HeLa total RNA 0.5 µg \/ 10 µL reaction\u003cbr\u003eExperiment 2: HeLa total RNA (0, 1 pg, 10 pg, 100 pg, 1 ng, 10 ng, 100 ng, 1 µg) + human gDNA 100 ng \/ 20 µL reaction\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReal-time PCR\u003c\/strong\u003e\u003cbr\u003eReagent: THUNDERBIRD™ SYBR® qPCR Mix (Code No. QPS-201)\u003cbr\u003eTemplate: cDNA 2 µL \/ 20 µL reaction (cDNA solution: 10 %)\u003cbr\u003eTarget: β-actin (188 bp)\u003cbr\u003eReal-time cycler: Applied Biosystems 7900HT\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/FSQ-301_20240528.pdf?v=1775789365\" target=\"_blank\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\"\u003e Download Manual \u003c\/a\u003e\n  \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/FSQ-301_MSDS.pdf?v=1775789364\" target=\"_blank\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\"\u003e Download SDS \u003c\/a\u003e\n  \u003ca href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" target=\"_blank\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\"\u003e\n                Request COA\n            \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Toyobo","offers":[{"title":"200Rxn","offer_id":52417372881077,"sku":"FSQ-301","price":277.57,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/ReverTra_Ace_qPCR_RT_Master_Mix_gDNA_Remover-Toyobo.jpg?v=1777517937","url":"https:\/\/biofargo.com\/products\/revertra-ace-qpcr-rt-master-mix-gdna-remover","provider":"Biofargo","version":"1.0","type":"link"}