{"product_id":"revertra-ace-qpcr-rt-kit","title":"ReverTra Ace qPCR RT Kit-Toyobo","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eReverTra Ace™ qPCR RT Kit is an efficient and convenient kit to synthesize high quality cDNAs for real-time PCR. This kit contains the highly efficient reverse transcriptase \"ReverTra Ace™\" and a RT buffer optimized for the highly efficient synthesis of short-chain cDNAs suitable for real-time PCR. The protocol is simple, and the reaction can be completed in 15 min.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0;\"\u003eReverTra Ace™ is a mutant-type M-MLV reverse transcriptase shows excellent efficiency.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0;\"\u003eReverTra Ace™ qPCR RT Master Mix (Code No. FSQ-201) is a premix version of ReverTra Ace™ qPCR RT Kit. The master mix reagent (5x) contains the highly efficient reverse transcriptase \"ReverTra Ace™\" , primers and buffer optimized for highly efficient synthesis of short-chain cDNAs suitable for real-time PCR.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding-left: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e The optimized RT buffer and Primer Mix enable the highly efficient reverse transcription.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e The reaction can be completed in 15 min. The protocol does not contain an additional RNase H treatment step to remove residual RNA after reverse transcription (Patent Pending).\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e Suitable for the detection of low-expressing mRNAs. Since the RT buffer is optimized for real-time PCR, the addition of 20% (v\/v) of the synthesized cDNA solution to the PCR solution does not inhibit the PCR reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eItem\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc;\"\u003eStorage condition\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eStore at -20ºC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"4\" style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc;\"\u003eReverTra Ace™ qPCR RT Kit\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e5 x RT Buffer\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e400 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eEnzyme Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e100 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003ePrimer Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e100 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eNuclease-free water\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1000 µL × 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc;\"\u003eReverTra Ace™ qPCR RT Master Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e5 x RT Master Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e400 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e5 x RT Master Mix no RT-Control\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e40 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eNuclease-free water\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1000 µL × 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication\u003c\/h2\u003e\n\u003cp style=\"padding-left: 5px;\"\u003eReverse transcriptation for real-time PCR\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication Data\u003c\/h2\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 1. Comparison of cDNA yields using various reverse transcription kits\u003c\/h3\u003e\n\u003cp\u003ecDNA was synthesized from human cell line (HeLa cell) total RNA with the ReverTra Ace™ qPCR RT kit. Subsequently, quantification of β-actin and polymerase ε mRNA were performed by real-time PCR with 100 ng cDNA, in conjunction with the SYBR® Green Realtime PCR Master Mix [Code No. QPK-201].\u003c\/p\u003e\n\u003cp\u003eAs shown in Fig. 1, the relative cDNA yields from the ReverTra Ace™ qPCR RT kit were greater than from the other commercial kits.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\n\u003cimg alt=\"Comparison of relative cDNA concentrations\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic01.png?v=1775200184\" style=\"max-width: 100%; height: auto;\"\u003e\n\u003cp style=\"font-size: 14px; color: #666; font-style: italic; margin-top: 10px;\"\u003e\u003cstrong\u003eFig. 1 Comparison of relative cDNA concentrations\u003c\/strong\u003e\u003cbr\u003eThe reaction temperature conditions of the reverse transcription are indicated under the figure.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 2. Detection of a rare expressing gene\u003c\/h3\u003e\n\u003cp\u003eSeveral 1-µL cDNA aliquots were prepared from 100 ng human cell line total RNA in a volume of 20 µL using various reverse transcription kits. Subsequently, a low expressing gene (TNF-α) was detected with real-time PCR kit (SYBR® Green Realtime PCR Master Mix [Code No. QPK-201]. The Ct values and success rates of the ReverTra Ace qPCR RT kit were greater than those from the other commercial kits (Fig. 2).\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\n\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic02.png?v=1775200185\" alt=\"Comparison of Ct values\" style=\"max-width: 100%; height: auto;\"\u003e\n\u003cp style=\"font-size: 14px; color: #666; font-style: italic; margin-top: 10px;\"\u003e\u003cstrong\u003eFig. 2 Comparison of Ct values and success rates when detecting rare expressing genes\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/FSQ-101_ee0e3772-9264-48aa-a882-96f73e39e32b.pdf?v=1775784063\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" target=\"_blank\"\u003e Download Manual \u003c\/a\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/FSQ-101_MSDS_4dc52fc2-649e-45aa-8c50-69d91e404814.pdf?v=1775784062\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e \u003ca href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" target=\"_blank\"\u003e Request COA \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Toyobo","offers":[{"title":"200Rxn","offer_id":52417371177141,"sku":"FSQ-101","price":248.77,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/FSQ-101.jpg?v=1777518036","url":"https:\/\/biofargo.com\/products\/revertra-ace-qpcr-rt-kit","provider":"Biofargo","version":"1.0","type":"link"}