Description

Respiratory Bacterial Pathogens Multiplex Probe Method qRT-PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

High sensitivity: analytical LOD as low as 100 copies/reaction
Excellent specificity: no cross-reactivity with DNA from other pathogens
Includes amplification positive control for result verification
Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.
Suitable for both qualitative and quantitative applications
For quantitative use: the linear range covers a 100,000-fold concentration difference.
Sufficient for 50 reactions (20 μL each)
For Research Use Only (RUO)

Kit Components

Component Name	Product Number.	Size	Vial	Lid Color
2×Probe qPCR MasterMix	981201	0.5 mL	0.5 mL Vial	Natural
Template Dilution Buffer	180701	1 mL	1.5 mL Vial	Green
Primer-Probe Powder	yp15-13740	50 T	0.5 mL Brown Vial	Brown
Amplification Positive Control (1E7 copies/μL)	pc13740	50 μL	0.5 mL Vial	Yellow
Ultrapure water	210806	1 mL	1.5 mL Vial	Blue
Manual	sc15-13740	1 Set	NA	NA

Application Areas

Marine aquaculture health monitoring: multiplex qPCR detection of respiratory bacterial pathogens in abalone, finfish and shellfish for early disease diagnosis and optimized stock management
Environmental pathogen surveillance: quantitative monitoring of bacterial contaminants in seawater and recirculating aquaculture systems to assess ecosystem health and inform biosecurity measures
Seafood safety testing: high-sensitivity screening of respiratory bacterial pathogens in harvested and processed marine products to ensure regulatory compliance and supply chain integrity

Storage & Shipping

Store at - 20 °C
The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.

Documents

Respiratory Bacterial Pathogens Multiplex Probe Method qRT-PCR Kit User Manual

MSDS