Description

SHENTEK® Residual Vero DNA-154 Quantitation Kit is used to quantitate residual Vero host DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit utilizes fluorescent quantitative PCR technique to perform a rapid, specific, and reliable quantitation assay at the femtogram (fg) level. The kit provides Vero DNA Control as reference standard. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).

Overview

Components	Shipping Condition	Unit Size	Detection Method
Vero DNA Control	-20℃	100 Reactions	Probe-based qPCR
qPCR Reaction Buffer	-20℃, protect from light
Vero Primer&Probe MIX-154	-20℃, protect from light
DNA Dilution Buffer (DDB)	-20℃

Specification

Range	3x10^-3-3x10¹ pg/μL, R²=0.99949，E=92.5%
Accuracy	Recovery = 87.7 %-98.5%, CV<30%
LOQ	0.003 pg/μL
LOD	0.0003 pg/μL
Precision	6.2%-18.3% (<30%)
Robustness	Freeze-thaw stability over 5 cycles
Robustness	Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments

Documents

User Guide

Related Products

SHENTEK® Residual Host Cell DNA Sample Preparation Kit

Residual Vero DNA-154 Quantitation Kit SHENTEK®