Description

SHENTEK® Residual Sf9&AcNPV DNA Quantitation Kit is used to quantitate residual Sf9&AcNPV host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products. This Sf9 test kit utilizes multiplex quantitative PCR (qPCR) technique to perform a rapid, specific, and reliable quantitation assay at the femtogram (fg) level. IPC (Internal Positive Control) is included in the assay to evaluate the performance of each PCR reaction. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).

Overview

Components	Shipping Condition	Unit Size	Detection Method
Sf9&AcNPV DNA Control	-20℃	100 Reactions	Probe-based qPCR
qPCR Reaction Buffer	-20℃, protect from light
Sf9&AcNPV Primer&Probe MIX
IPC MIX
DNA Dilution Buffer (DDB)	-20℃

Specification

Range	Sf9: 3x10^-3-3x10² pg/μL, R²=0.99950，E=96.5%
	AcNPV: 5.14x10¹ - 5.14x10⁶ copies/μL, R²=0.99952，E=93.0%
Accuracy	Sf9: Recovery = 80.0%-129.0%, CV<30%
	AcNPV: Recovery = 80.4%-85.9%, CV<30%
LOQ	Sf9: 0.0015 pg/μL
	AcNPV: 51.4 copies/μL
LOD	Sf9: 0.0003 pg/μL
	AcNPV: 1.71 copies/μL
Precision	Sf9: 6.0%-9.8% (<30%)
	AcNPV: 4.1%-6.8% (<30%)
Robustness	Freeze-thaw stability over 5 cycles
	Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments

Documents

User Guide

Related Products

SHENTEK™ Residual Host Cell DNA Sample Preparation Kit

Residual Sf9 & AcNPV DNA Quantitation Kit SHENTEK®