Description

SHENTEK® Residual Pichia Pastoris DNA Quantitation Kit is used to quantitate residual Pichia Pastoris DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit utilizes fluorescent quantitative PCR technique (FAM) to perform rapid and specific quantitation of residual Pichia Pastoris DNA fragments in samples, and reliable quantitation assay at the fg level. Pichia Pastoris DNA Control is provided as reference standard. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).

Overview

Components	Shipping Condition	Unit Size	Detection Method
Pichia Pastoris DNA Control	-20℃	100 Reactions	Probe-based qPCR
Pichia Pastoris qPCR Reaction Buffer	-20℃, protect from light
Pichia Pastoris Primer&Probe MIX	-20℃, protect from light
DNA Dilution Buffer (DDB)	-20℃

Specification

Range	3x10^-2-3x10² pg/μL, R²=0.99976，E=95.0%
Accuracy	Recovery = 78.8%~110.6%, CV < 30%
LOQ	0.03 pg/μL
LOD	0.001 pg/μL
Precision	4.3%-8.8% (<30%)
Robustness	Freeze-thaw stability over 5 cycles
Robustness	Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments

Documents

User Guide

Related Products

SHENTEK® Residual Host Cell DNA Sample Preparation Kit

Residual Pichia pastoris DNA Quantitation Kit SHENTEK™