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Description
Residual PG13 DNA Size Analysis Kit is used to quantitate PG13 host DNA residues of different fragment sizes in different stages of biopharmaceutical products, from in-process samples to final products.
The SHENTEK® Residual PG13 DNA Size Analysis Kit utilizes fluorescent quantitative PCR technique (FAM) to perform rapid and specific quantitation of residual PG13 DNA fragments in samples. The assay is designed to amplify three different fragments (118bp, 212bp, 502bp) for determining the accurate size distribution, and the limit of detection (LOD) reaches femtograms (fg) level.
For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
Size: 3 × 100 Reactions
Introduction
| Components | Shipping Condition | Unit Size | Detection Method |
| BHK DNA Control | -20℃ | 3×100 Reactions | Probe-based qPCR |
| qPCR Reaction Buffer | -20℃, protect from light | ||
| PG13 Primer&Probe MIX-118 | |||
| PG13 Primer&Probe MIX-212 | |||
| PG13 Primer&Probe MIX-502 | |||
| IPC MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ |
Specification
| Range | St1~St5：0.03~300 pg/μL（3 ng/rxn~300 fg/rxn）,R2>0.990，83.3%≤E≤110.0%。 |
| LOQ | 0.03 pg/μL（300 fg/rxn） |
| Precision | CV<40% |
| Robustness | Freeze-thaw stability over 4 cycles |
| Instrument suitability not limited to Thermo, SHENTEK qPCR equipments |
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When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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