Description

Residual NS0 & SP2/0 DNA Quantitation Kit is used to quantitate residual NS0&SP2/0 host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products.

The SHENTEK® Residual NS0&SP2/0 DNA Quantitation Kit utilizes quantitative PCR (qPCR) technique to perform a rapid, specific, and reliable quantitation assay at the femtogram (fg) level. The kit provides NS0&SP2/0 DNA Control as reference standard. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).

Introduction

Components	Shipping Condition	Unit Size	Detection Method
NS0&SP2/0 DNA Control	-20℃	100 Reactions	Probe-based qPCR
qPCR Reaction Buffer	-20℃, protect from light
NS0&SP2/0 Primer&Probe MIX	-20℃, protect from light
DNA Dilution Buffer (DDB)	-20℃

Specification

Range	3 fg/μL~300 pg/μL, R²=0.999, E=93.4%
Accuracy	Recovery = 75.8%-107.7% , CV<15%
LOQ	3 fg/μL
Precision	CV<15%
Robustness	Freeze-thaw stability over 4 cycles
Robustness	Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments

Documents

User Guide

Related Products

SHENTEK® Residual Host Cell DNA Sample Preparation Kit

Residual NS0 & SP2/0 DNA Quantitation Kit SHENTEK®