Description

SHENTEK® Residual MDCK DNA Quantitation Kit is used to quantitate residual MDCK host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit utilizes quantitative PCR (qPCR) technique to a perform rapid, specific, and reliable quantitation assay at the femtogram (fg) level. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).

Overview

Components	Shipping Condition	Unit Size	Detection Method
MDCK DNA Control	-20℃	100 Reactions	Probe-based qPCR
qPCR Reaction Buffer	-20℃, protect from light
MDCK Primer&Probe MIX	-20℃, protect from light
DNA Dilution Buffer (DDB)	-20℃

Specification

Range	3x10^-3-3x10² pg/μL, R²=0.999，E=98.2%
Accuracy	Recovery = 68.0%-74.0%, CV<30%
LOQ	0.003 pg/μL
Precision	6.6%-12.7% (<30%)
Robustness	Freeze-thaw stability over 5 cycles
Robustness	Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments

Documents

User Guide

Related Products

SHENTEK® Residual Host Cell DNA Sample Preparation Kit

Residual MDCK DNA Quantitation Kit SHENTEK®