Description

SHENTEK™ Residual HEK293 DNA Quantitation Kit is used to quantitate residual HEK293 host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit uses duplex real-time PCR technology to performs rapid, specific, and reliable quantitation assay at the fg level. IPC(Internal Positive Control) is included in the HEK293 Primer&Probe MIX to evaluate the performance of each PCR reaction. For extraction information, please refer to the SHENTEK™ Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).

Introduction

Components	Shipping Condition	Unit Size	Product Line	Detection Method
CHO DNA Control	-20℃	100 Reactions	SHENTEK™	Probe-based qPCR
qPCR Master MIX
DNA Dilution Buffer (DDB)
CHO Primer&Probe MIX (Incl IPC)	-20℃, protect from light

Specification

Range	3x10-2-3x102 pg/μL, R2=0.99984, AE=98.5%
Accuracy	Recovery = 82.1%-104.0%, CV<30%
LOQ	0.03 pg/μL
Precision	8.9%-15.8% (<30%)
Robustness	Freeze-thaw stability over 5 cycles
Robustness	Instrument suitability not limited to Thermo, Biorad, Roche, HZSKBio qPCR equipments

Documents

User Guide

Related Products

SHENTEK™ Residual Host Cell DNA Sample Preparation Kit

Residual HEK293 DNA Quantitation Kit (3G) SHENTEK™