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Description
Residual E. coli DNA Quantitation Kit is used to quantitate residual E. coli host cell DNA in different stages of biopharmaceutical products. IPC (Internal Positive Control) is included in the E. coli Primer&Probe MIX to evaluate the performance of each PCR reaction.
SHENTEK® Residual E. coli DNA Quantitation Kit is used to quantitate residual E. coli host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit uses duplex real-time PCR technology to perform rapid, specific, and reliable quantitation assay at the fg level. IPC (Internal Positive Control) is included in the E. coli Primer&Probe MIX to evaluate the performance of each PCR reaction. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
Introduction
| Components | Shipping Condition | Unit Size | Detection Method |
| E. coli DNA Control | -20℃ | 100 Reactions | Probe-based qPCR |
| qPCR Master MIX | |||
| DNA Dilution Buffer (DDB) | |||
| E. coli Primer&Probe MIX (Incl IPC) | -20℃, protect from light |
Specification
| Range | 3x10-2-3x102 pg/μL, R2=0.99968, E=97.5% |
| Accuracy | Recovery = 81.2%-107.4% , CV<30% |
| LOQ | 0.03 pg/μL |
| LOD | 0.003 pg/μL |
| Precision | 2.8%-17.9% (<30%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
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When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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