Description

SHENTEK™ Residual CHO DNA Quantitation Kit is used to quantitate host-cell line residual DNA from CHO cell line, which is used for production of biopharmaceutical products. This kit uses duplex real-time PCR technology to performs rapid, specific, and reliable quantitation assay at the fg level. IPC (Internal Positive Control) is included in the CHO Primer&Probe MIX to evaluate the performance of each PCR reaction. For extraction information, please refer to the SHENTEK™ Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).

Introduction

Components	Shipping Condition	Unit Size	Product Line	Detection Method
CHO DNA Control	-20℃	100 Reactions	SHENTEK™	Probe-based qPCR
qPCR Master MIX
DNA Dilution Buffer (DDB)
CHO Primer&Probe MIX (Incl IPC)	-20℃, protect from light

Specification

Range	3x10-3-3x102 pg/μL, R2=0.99939，E=94.7%
Accuracy	Recovery = 80.2 %-101.6%, CV<30%
LOQ	0.003 pg/μL
LOD (LLOQ)	0.0003 pg/μL
Precision	5.2%-22.5% (<30%)
Robustness	Freeze-thaw stability over 5 cycles
Robustness	Instrument suitability not limited to Thermo, Biorad, Roche, HZSKBio qPCR equipments

Documents

User Guide

Related Products

SHENTEK™ Residual Host Cell DNA Sample Preparation Kit

Residual CHO DNA Quantitation Kit (2G) SHENTEK™