Recombinant R-spondin 1,Human,AF,HEK293

Key features and details:

• Expression System:HEK293
• Purity/method:>95% as determined by SDS-PAGE. Ni-NTA chromatography
• Fusion tag:His-SUMO tag at the N-terminus
• Application: WB, ELISA, SDS-PAGE, Cell culture, Elisa

Product Description:

Protein Description:

R-spondin 1 (RSPO1, Roof plate-specific Spondin 1) is a cysteine-rich secreted glycoprotein that belongs to the R-Spondin family. Structurally, R-spondin 1 harbors an N-terminal signal peptide, two cysteine-rich furin-like (FU1-FU2) domains, a thrombospondin (TSP) domain, and a basic amino acid-rich (BR) C-terminal domain. R-spondin 1 acts as a ligand for the leucine-rich repeat-containing G-protein coupled receptor (LGR) family (LGR4/5/6), subsequent clearing negative regulators ZNRF3/RNF43 from the membrane, leading to the availability of Wnt receptor and activating Wnt/ض-catenin signaling. The signal cascade is essential for regulating stem cell proliferation, embryonic development, and tissue homeostasis.

Protein Accession: Q2MKA7

Gene ID: 284654

Species: Human

Expression Sequence:

A DNA sequence encoding Human R-Spondin 1 Protein (#Q2MKA7) (Ser21 - Ala263) was expressed with polyhistidine-SUMO tag at the N-terminus.

Activity:

Testing in process

Endotoxin level: <1 EU per 1 ug of the protein by the LAL method.

Calculated Molecular Weight: 42.43 kDa

Formulation: Liquid (1X PBS, pH 7.4)

Reconstitution:

N/A

Shipping: Blue Ice

Stability and Storage:

Store at 2 degrees Celsius to 8 degrees Celsius for up to 2 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20 degrees Celsius or -80 degrees Celsius for 3-6 months.

Category: Cytokines

SDS-PAGE Image Name: human R-spondin 1

Datasheets and documents: