Recombinant IGFBP7, Human,His-SUMO Tag, HEK293

Key features and details:

• Expression System:HEK293
• Purity/method:>95% as determined by SDS-PAGE. Ni-NTA chromatography.
• Fusion tag:His-SUMO tag at the N-terminus
• Application: WB, ELISA, SDS-PAGE, Cell culture, Elisa

Product Description:

Protein Description:

IGFBP7, also known as mac25, prostacyclin-stimulating factor (PSF), tumor adhesion factor (TAF), and angiomodulin (AGM), is a secreted protein that belongs to the insulin-like growth factor (IGF)-binding protein (IGFBP) family. In contrast to the other family members, IGFBP7 binds IGFs through the N-terminal domain with low affinity. IGFBP7 is expressed in various human tissues, including the brain, liver, pancreas, and skeletal muscle, and is secreted into circulation. IGF-BP7 modulates many biological functions, such as protein synthesis, proliferation, anti-apoptosis, and cell survival. Moreover, it has been reported that IGFBP7 promotes cardiac senescence through IGF-1R/IRS/AKT-dependent suppression of FOXO3a, inhibiting DNA repair, subsequently the leading to the progression of Heart failure. Human and mouse IGFBP7 are highly homologous, sharing 94% a.a. sequence identity.

Protein Accession: Q16270

Gene ID: 3490

Species: Human

Expression Sequence:

A DNA sequence encoding Human IGFBP7 (#Q16270) (Ser27 - Leu282) was expressed with polyhistidine-SUMO tag at the N-terminus.

Activity:

Testing in process

Endotoxin level: <1 EU per 1 ug of the protein by the LAL method.

Calculated Molecular Weight: 38.18 kDa

Formulation: The protein was lyophilized from a solution containing 1X PBS, pH 7.4.

Reconstitution:

It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 ug/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Shipping: Blue Ice

Stability and Storage:

Lyophilized protein should be stored at -20 degrees Celsius for 1 year. Upon reconstitution, store at 2 degrees Celsius to 8 degrees Celsius for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20 degrees Celsius or -80 degrees Celsius for 3-6 months.

Category: Cytokines

SDS-PAGE Image Name: human IGFBP7

Datasheets and documents: