Specifications

Parameter	Description
Cat. No.	Y04701
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	Endotoxin
Bioactivity	Measured in a cell proliferation assay using HUVEC human umbilicveinendothelial cells. The ED50 for this effect is 0.2-5 ng/mL.
Accession	P15692
Alternative Names	Vascular Endothelial Growth Factor Isoform 165; VEGF165
Species	Human
Expression System	CHO
Mol. Weight	19.1kDa
Expression Region	Ala27-Arg191
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state;
6 months at -20°C to -80°C under sterile conditions after reconstitution;
7-10 days at 2°C to 8°C under sterile conditions after reconstitution;
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Data

SDS-PAGE

Recombinant Human VEGF165 (Cat. No. Y04701) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y04701 SDS-PAGE.

Bioactivity

Recombinant Human VEGF165 (Cat. No. Y04701) stimulates proliferation of HUVEC human umbilicvein endothelial cells. The ED50 for this effect is 0.2-10 ng/mL. Y04701 was higher than the other competing product.

Documents

VEGF165

Recombinant Human VEGF165 95%