Description

VEGF165 (Vascular Endothelial Growth Factor 165), one of the major isoforms of VEGF-A, functions as a disulfide-linked homodimer and plays a critical role in angiogenesis and cell culture. It activates the VEGFR2/PI3K/AKT signaling pathway to inhibit apoptosis and is commonly used to sustain the proliferative capacity of human umbilical vein endothelial cells (HUVECs) and microvascular endothelial cells in vitro. In Matrigel-based tube formation assays, VEGF165 stimulates endothelial cells to form tubular structures, thereby mimicking the process of angiogenesis. In stem cell differentiation systems, it can induce commitment to endothelial cell lineages or support the development of vascular-like structures.

Specifications

Product Details
Cat. No.	Y04701
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using HUVEC human umbilicveinendothelial cells. The ED50 for this effect is 0.2-5 ng/mL.
Accession	P15692
Alternative Names	Vascular Endothelial Growth Factor Isoform 165; VEGF165
Species	Human
Expression System	CHO
Mol. Weight	19.1kDa
Expression Region	Ala27-Arg191
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Supports hematopoietic stem cell niche and endothelial-hematopoietic interactions.

Immunology

Enhances immune cell migration via vascular modulation.

Stem Cell Research

Widely used to induce vascularization and endothelial differentiation in stem cell/organoid systems.

Cell Biology

Potent regulator of angiogenesis, vascular permeability, and endothelial survival.

Data Figures

Figure 1: Recombinant Human VEGF165 (Cat. No. Y04701) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y04701 SDS-PAGE.

Recombinant Human VEGF165 (Cat. No. Y04701) stimulates proliferation of HUVEC human umbilicvein endothelial cells. The ED50 for this effect is 0.2-10 ng/mL. Y04701 was higher than the other competing product.

Figure 2: Recombinant Human VEGF165 (Cat. No. Y04701) stimulates proliferation of HUVEC human umbilicvein endothelial cells. The ED50 for this effect is 0.2-10 ng/mL. Y04701 was higher than the other competing product.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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