{"product_id":"recombinant-human-vegf165-1","title":"Recombinant Human VEGF165","description":"\u003cstyle\u003e.biofargo-table-wrapper {overflow-x: auto;margin: 20px 0;-webkit-overflow-scrolling: touch;}.biofargo-spec-table {width: 100%;border-collapse: collapse;border: 1px solid #dee2e6;min-width: 600px;background: #fff;}.biofargo-spec-table th,.biofargo-spec-table td {border: 1px solid #dee2e6;padding: 12px;vertical-align: middle;white-space: normal;word-break: break-word;}@media (max-width: 600px) {.biofargo-table-wrapper {border: 1px solid #eee;border-radius: 8px;padding: 6px;background: #fff;}.biofargo-spec-table {min-width: 720px;}}\u003c\/style\u003e\u003cdiv style=\"font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1000px; margin: 0 auto; border: 1px solid #e1e8ed; padding: 30px; border-radius: 10px; background-color: #ffffff;\"\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 0; font-size: 22px;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #fcfcfc; padding: 15px; border-radius: 6px; border: 1px solid #f0f0f0;\"\u003e\u003cp style=\"margin: 0; text-align: justify;\"\u003eVEGF165 (Vascular Endothelial Growth Factor 165), one of the major isoforms of VEGF-A, functions as a disulfide-linked homodimer and plays a critical role in angiogenesis and cell culture. It activates the VEGFR2\/PI3K\/AKT signaling pathway to inhibit apoptosis and is commonly used to sustain the proliferative capacity of human umbilical vein endothelial cells (HUVECs) and microvascular endothelial cells in vitro. In Matrigel-based tube formation assays, VEGF165 stimulates endothelial cells to form tubular structures, thereby mimicking the process of angiogenesis. In stem cell differentiation systems, it can induce commitment to endothelial cell lineages or support the development of vascular-like structures.\u003c\/p\u003e\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrapper\"\u003e\u003ctable class=\"biofargo-spec-table\"\u003e\n\u003cthead\u003e\u003ctr style=\"background-color: #0056b3; color: white;\"\u003e\u003cth style=\"padding: 12px; text-align: left;\" colspan=\"2\"\u003eProduct Details\u003c\/th\u003e\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eCat. No.\u003c\/td\u003e\n\u003ctd\u003eY04701\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003ePurity\u003c\/td\u003e\n\u003ctd\u003e≥95%, by SDS-PAGE (under reducing (R) \u0026amp; Non-reducing conditions, visualized by Coomassie staining).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eEndotoxin\u003c\/td\u003e\n\u003ctd\u003e≤10 EU\/mg by the LAL method.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eBioactivity\u003c\/td\u003e\n\u003ctd\u003eMeasured in a cell proliferation assay using HUVEC human umbilicveinendothelial cells. The ED50 for this effect is 0.2-5 ng\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eAccession\u003c\/td\u003e\n\u003ctd\u003eP15692\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eAlternative Names\u003c\/td\u003e\n\u003ctd\u003eVascular Endothelial Growth Factor Isoform 165; VEGF165\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eSpecies\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eExpression System\u003c\/td\u003e\n\u003ctd\u003eCHO\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eMol. Weight\u003c\/td\u003e\n\u003ctd\u003e19.1kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eExpression Region\u003c\/td\u003e\n\u003ctd\u003eAla27-Arg191\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eTag Info\u003c\/td\u003e\n\u003ctd\u003eTag free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eFormulation\u003c\/td\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eBuffer\u003c\/td\u003e\n\u003ctd\u003ePBS, 5% Mannitol and 0.01% Tween 80, pH7.4.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eSize\u003c\/td\u003e\n\u003ctd\u003e10μg\/50μg\/1mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eLead Time\u003c\/td\u003e\n\u003ctd\u003eProducts are in stock, but custom products may require further consultation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eReconstitution\u003c\/td\u003e\n\u003ctd\u003eIt is recommended to redissolve in sterile deionized water.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eThe product is shipped with blue ice.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eStorage \u0026amp; Stability\u003c\/td\u003e\n\u003ctd\u003e36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eHigh Purity:\u003c\/strong\u003e This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eHigh Biological Activity:\u003c\/strong\u003e Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eBatch Consistency:\u003c\/strong\u003e Manufactured under strict production and quality control protocols to ensure highconsistency between batches.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eAnimal-Free:\u003c\/strong\u003e Produced without the use of animal-derived components, meeting animal-free requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eApplications\u003c\/h2\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 20px;\"\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eHematology\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eSupports hematopoietic stem cell niche and endothelial-hematopoietic interactions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eImmunology\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eEnhances immune cell migration via vascular modulation.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eStem Cell Research\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eWidely used to induce vascularization and endothelial differentiation in stem cell\/organoid systems.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eCell Biology\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003ePotent regulator of angiogenesis, vascular permeability, and endothelial survival.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eData Figures\u003c\/h2\u003e\n\u003cdiv style=\"text-align: center; margin-top: 20px; border: 1px solid #eee; padding: 15px; border-radius: 8px;\"\u003e\n\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"Recombinant Human VEGF165 (Cat. No. Y04701) SDS-PAGE under reducing (R) \u0026amp; Non-reducing conditions. The gel was stained with Y04701 SDS-PAGE.\" src=\"https:\/\/files.biofargo.com\/eastmabbio\/product-detail-images\/Y04701_1.jpg\"\u003e\u003cp style=\"font-size: 13px; color: #888; margin-top: 10px;\"\u003eFigure 1: Recombinant Human VEGF165 (Cat. No. Y04701) SDS-PAGE under reducing (R) \u0026amp; Non-reducing conditions. The gel was stained with Y04701 SDS-PAGE.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"text-align: center; margin-top: 20px; border: 1px solid #eee; padding: 15px; border-radius: 8px;\"\u003e\n\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"Recombinant Human VEGF165 (Cat. No. Y04701) stimulates proliferation of HUVEC human umbilicvein endothelial cells. The ED50 for this effect is 0.2-10 ng\/mL. Y04701 was higher than the other competing product.\" src=\"https:\/\/files.biofargo.com\/eastmabbio\/product-detail-images\/Y04701_2.jpg\"\u003e\u003cp style=\"font-size: 13px; color: #888; margin-top: 10px;\"\u003eFigure 2: Recombinant Human VEGF165 (Cat. No. Y04701) stimulates proliferation of HUVEC human umbilicvein endothelial cells. The ED50 for this effect is 0.2-10 ng\/mL. Y04701 was higher than the other competing product.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eInstructions for Use\u003c\/h2\u003e\n\u003cul style=\"padding-left: 20px;\"\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eDissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003ePrepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eDo not mix with other similar products to avoid cross-reactivity and potential interference.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eCautions\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #fff5f5;  padding: 15px; color: #852d2d; font-size: 14px;\"\u003e\n\u003cp style=\"margin: 0 0 8px 0;\"\u003e\u003cstrong\u003e• Research Use Only:\u003c\/strong\u003e This product is not suitable for clinical diagnostics or therapeutic applications.\u003c\/p\u003e\n\u003cp style=\"margin: 0 0 8px 0;\"\u003e\u003cstrong\u003e• Preservation:\u003c\/strong\u003e Handle according to instructions to preserve its activity and stability.\u003c\/p\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cstrong\u003e• Support:\u003c\/strong\u003e Contact our technical support team if you encounter any issues or questions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"display: flex; gap: 15px; margin-top: 10px; flex-wrap: wrap;\"\u003e\n\u003ca style=\"display: inline-block; padding: 10px 20px; border: 2px solid #0056b3; color: #0056b3; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/files.biofargo.com\/eastmabbio\/coa\/20250609-Y04701-Recombinant%20Human%20VEGF165-10%CE%BCg-%E5%85%A8%E8%8B%B1%E7%89%88..pdf\" target=\"_blank\"\u003e📄 Download COA\u003c\/a\u003e \u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003egmp-tl612-50\u003c\/div\u003e","brand":"EastMabBio","offers":[{"title":"10ug","offer_id":52402235015349,"sku":"Y04701-10ug","price":94.0,"currency_code":"USD","in_stock":true},{"title":"50ug","offer_id":52284501852341,"sku":"Y04701-50ug","price":330.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":52284501885109,"sku":"Y04701-1mg","price":2442.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Y04701.jpg?v=1771997967","url":"https:\/\/biofargo.com\/products\/recombinant-human-vegf165-1","provider":"Biofargo","version":"1.0","type":"link"}