Description

Recombinant Human Thrombopoietin (TPO) is a key hematopoietic growth factor that regulates the production, differentiation, and maturation of megakaryocytes and platelets. It exerts its biological effects by binding to the c-Mpl receptor, activating downstream signaling pathways such as JAK/STAT, MAPK, and PI3K/AKT, which are essential for hematopoietic stem cell maintenance and platelet formation.

This recombinant protein is expressed in CHO cells and corresponds to the functional region of human TPO (Ser22–Leu195), ensuring proper folding and biological activity. With high purity (≥95%) and strong bioactivity (ED50 ≤1.0 ng/mL in Mo7e cell proliferation assay), this product is suitable for research in hematology, stem cell biology, and cytokine signaling. The protein is supplied in a tag-free, lyophilized form for convenient storage and reconstitution.

Specifications

Product Details
Cat. No.	Y07201
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin	≤10 EU/mg by the LAL method
Bioactivity	Measured in a cell proliferation assay using Mo7e human leukemia cell line. The ED50 for this effect is ≤1.0 ng/mL.
Accession	P40225
Alternative Names	rHuThrombopoietin/TPO; TPO
Species	Human
Expression System	CHO
Mol. Weight	18.6 kDa
Expression Region	Ser22–Leu195
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size	10μg/50μg/1mg
Lead Time	Products are in stock,but custom products may require further consultation
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.
✔ High Biological Activity: Exhibits potent activity with ED50 ≤1.0 ng/mL in Mo7e cell proliferation assay, ensuring consistent performance.
✔ Mammalian Expression System: Expressed in CHO cells to ensure correct folding and native-like biological functionality.
✔ Convenient and Reliable: Supplied in a tag-free, lyophilized form for easy reconstitution, stable storage, and compatibility with diverse experimental applications.

Applications

Hematology

Regulates megakaryocyte development and platelet production, widely used in hematopoietic research.

Stem Cell Research

Supports maintenance and expansion of hematopoietic stem and progenitor cells.

Cell Biology

Used to study cytokine signaling pathways such as JAK/STAT and mechanisms of cell proliferation and differentiation.

Data Figures

Measured in a cell proliferation assay using Mo7e cells. The ED50 for this effect is ≤1.0 ng/mL. The Bioactivity of Y07201 was higher than other competing products.

Figure 1: Measured in a cell proliferation assay using Mo7e cells. The ED₅₀ for this effect is ≤1.0 ng/mL. The Bioactivity of Y07201 was higher than other competing products.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download

Recombinant Human TPO