Description

Recombinant Human Thrombopoietin (TPO) is a functional protein transiently expressed in HEK-293 cells with a C-terminal 6×His tag.

By retaining the N-terminal 1–174 amino acid residues of TPO (the EPO-homologous domain essential for bioactivity) and removing the non-essential C-terminal glycosylated region, its molecular weight is optimized to 18–23 kDa.

This protein specifically binds to the c-Mpl receptor and activates the JAK/STAT signaling pathway, playing a central role in hematopoietic stem cell culture and cell therapy applications.

It drives directed differentiation of hematopoietic stem/progenitor cells toward the megakaryocytic lineage, efficiently promoting in vitro expansion and maturation of megakaryocytes, as well as platelet production.

This provides critical support for establishing functional in vitro platelet production platforms, developing thrombocytopenia therapeutics, and advancing stem cell-based regenerative therapies.

Specifications

Parameter	Specification	Results
Appearance	Freeze-dried preparation, the form should be white loose body	Compliance
Identity	The electrophoretic bands should be consistent with the molecular weight of the product	Compliance
Visible Particle	Should be no visible particle	Compliance
Moisture	≤4.0%	2.8%
pH	6.5~7.5	7.3
Purity	>90.0%	Compliance
Endotoxin	<0.1EU/μg	<0.1EU/μg
Mycoplasma	Should be negative	Negative
Sterility	No growth	No growth
Biological Activity	Cell proliferation assay performed with Mo7e cells demonstrated specific activity ≥ 5.0 × 10⁶ IU/mg	3.13 × 10⁷ IU/mg

Features

✔ HEK293-Expressed Truncated TPO (Ser22–Leu195)
Recombinant human TPO expressed in HEK293 cells, corresponding to Ser22–Leu195 with Pro157Asn and Arg164Asn substitutions, retaining the EPO-homologous bioactive domain and optimized molecular weight (~19.5 kDa), with a C-terminal 6×His tag.
✔ High Purity and Controlled Quality Attributes
Purity >90% by SDS-PAGE, electrophoretic identity consistent with expected molecular weight, no visible particles, moisture 2.8%, pH 7.3 within 6.5–7.5 specification.
✔ Low Endotoxin and Sterility Assurance
Endotoxin <0.1 EU per µg (LAL method), mycoplasma negative, and sterility confirmed with no growth.
✔ Validated Mo7e Proliferation Activity
Biological activity measured by Mo7e cell proliferation assay, with specific activity ≥5.0 × 10⁶ IU/mg (measured 3.13 × 10⁷ IU/mg).

Applications

HSPC Culture Support

Binds to c-Mpl receptor and activates JAK/STAT signaling to support hematopoietic stem/progenitor cell expansion.

Megakaryocytic Differentiation

Drives directed differentiation of hematopoietic stem/progenitor cells toward the megakaryocyte lineage in vitro.

Megakaryocyte Maturation Studies

Promotes in vitro expansion and maturation of megakaryocytes for platelet generation studies.

In Vitro Platelet Platforms

Supports development of functional in vitro platelet production systems and stem cell–based regenerative research.

Data Figures

Recombinant Human Thrombopoietin (TPO) Protein

Stability & Storage

Lyophilized powder: Stable for 12 months at −80 °C or 6 months at −20 °C when stored in the original sealed container under desiccant.
Reconstitution: Dissolve in sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration ≥100 µg/mL to prevent adsorption.
Handling: Aliquot to avoid repeated freeze–thaw cycles.

Cautions

• Research Use Only: This product is not intended for diagnostic or therapeutic use.

• Handling: Prepare solutions using sterile technique. Avoid microbial contamination to maintain activity.

• Storage: Follow recommended storage conditions. Repeated freeze–thaw cycles may reduce performance.

Documents

📄 TL-650 Data Sheet 📄 TL-650 SDS 📄 TL-650 COA

Recombinant Human TPO Protein