Description

TNF-α (Tumor Necrosis Factor-α) is an important cytokine primarily produced by activated immune cells such as macrophages, lymphocytes, and NK cells. It plays a central role in physiological and pathological processes including inflammation, immune regulation, and apoptosis. As a pro-inflammatory cytokine, TNF-α participates in normal inflammatory and immune responses. It orchestrates tissue homeostasis by coordinately regulating the production of other cytokines, as well as modulating cell survival and death. TNF-α exerts diverse functions in various developmental and immune processes—such as inflammation, differentiation, lipid metabolism, and apoptosis—and is implicated in numerous diseases.

Specifications

Product Details
Cat. No.	Y04401
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 5-60 pg/mL.
Accession	P01375
Alternative Names	umor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2
Species	Human
Expression System	CHO
Mol. Weight	17.4 kDa
Expression Region	Val77-Leu233
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Modulates hematopoietic progenitor apoptosis and differentiation under stress.

Immunology

Key pro-inflammatory cytokine, regulates immune responses.

Stem Cell Research

Used to mimic inflammatory microenvironments affecting stem cell fate.

Cell Biology

Regulates apoptosis, proliferation, and inflammation signaling.

Data Figures

Figure 1: Recombinant Human TNFα (Cat. No. Y04401) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y04401 SDS-PAGE.

Recombinant Human TNFα (Cat. No. Y04401) measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 5-60 pg/mL. The bioactivity of Y04401 was equivalent to other competing products.

Figure 2: Recombinant Human TNFα (Cat. No. Y04401) measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 5-60 pg/mL. The bioactivity of Y04401 was equivalent to other competing products.

Figure 3: Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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Recombinant Human TNF-α