Description

Tissue Factor is a transmembrane glycoprotein that initiates the extrinsic coagulation cascade by binding factor VII/VIIa. In in vitro systems, TF is used in coagulation studies, vascular biology, and disease models involving thrombosis and inflammation.

Specifications

Product Details
Cat. No.	Y04301
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC . The AC50 is < 5 μg/mL.
Accession	P13726
Species	Human
Expression System	CHO
Mol. Weight	24.8 kDa
Expression Region	S33-E251
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Initiates coagulation cascades, indirectly influencing hematopoietic niche.

Cell Biology

Regulates coagulation and cell signaling related to hemostasis.

Data Figures

Figure 1: Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC . The AC50 is < 5 μg/mL.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human Tissue Factor