Description

TGF-β3 (Transforming Growth Factor-β3) is a pleiotropic cytokine within the TGF-β superfamily that plays important roles in embryonic development, tissue repair, immune regulation, and cell differentiation. Unlike TGF-β1, which is pro-fibrotic, TGF-β3 exhibits unique functions in wound healing and embryonic development—particularly a stronger anti-scarring capacity. Similar to TGF-β1, TGF-β3 can inhibit the proliferation of many epithelial cells and promote epithelial-to-mesenchymal transition (EMT), which is significant in cancer research and developmental models. In stem cell culture, TGF-β3—often used in combination with BMP—induces the differentiation of mesenchymal stem cells (MSCs) into chondrocytes, making it valuable for cartilage tissue engineering.

Specifications

Product Details
Cat. No.	Y07901
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2 ng/mL.
Accession	P10600
Alternative Names	Transforming growth factor beta-3; TGFB3; TGF-beta-3; Latency-associated peptide; LAP
Species	Human
Expression System	CHO
Mol. Weight	13 kDa
Expression Region	Ala 301-Ser 412
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing 100 mM Glycine, 150 mM NaCl, 5% Mannitol and 0.01% Tween 80, pH 4.0.
Size	10μg/50μg/1mg
Lead Time	Products are in stock,but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Modulates stromal support for hematopoiesis.

Immunology

Participates in immune regulation, less potent than TGF-β1.

Stem Cell Research

Influences stem cell differentiation and tissue remodeling.

Cell Biology

Regulates ECM deposition, proliferation, and differentiation processes.

Data Figures

Measured in a cell inhibitionn assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2 ng/mL. The bioactivity of Y07901 was higher than other competing products.

Figure 1: Measured in a cell inhibitionn assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2 ng/mL. The bioactivity of Y07901 was higher than other competing products.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human TGF-β3