Description

Recombinant Human TGF-β2 is produced using a specific mammalian cell line. This product corresponds to the Human TGF-β2 sequence from Ala303 to Ser414 (Accession # P61812). It has a predicted molecular mass of 12.7 kDa as a monomer.

Specifications

Product Details
Cat. No.	Y07801N
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.3ng/mL.
Accession	P61812
Alternative Names	Transforming growth factor beta-2; TGFB2; Polyergin; G-TSF; Glioblastoma-derived T-cell suppressor factor; Cetermin; BSC-1 cell growth inhibitor; TGF-beta-2
Species	Human
Expression System	CHO
Mol. Weight	12.7 kDa
Expression Region	Ala 303-Ser 414
Tag Info	Tag free
Formulation	Lyophilized
Buffer	0.085% TFA, 30% ACN, 5% Mannitol, pH 2.5.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in 4 mM HCL.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: Purity is 95% or higher, verified by SDS-PAGE for reliable results.
✔ High Biological Activity: Strong potency with an ED50 of 0.3 ng/mL or less in cell inhibition assays.
✔ Batch Consistency: Rigorous manufacturing ensures stable performance and reproducible bioactivity across lots.
✔ Animal-Free: Produced in a mammalian CHO cell line and 0.22 micron sterile-filtered.

Applications

Hematology

Study of blood cell proliferation and inhibitory regulatory mechanisms.

Immunology

Research on cytokine signaling pathways and immune system modulation.

Stem Cell Research

Support for cell differentiation and lineage specification in sterile cultures.

Cell Biology

Investigation of fundamental cellular growth, apoptosis, and signal transduction.

Data Figures

Figure 1: Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.3 ng/mL.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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