Description

TGF-β1 (Transforming Growth Factor-β1) is a multifunctional cytokine belonging to the TGF-β superfamily. It inhibits the growth of most epithelial, endothelial, and hematopoietic cells by inducing cell cycle arrest in the G1 phase. In stem cell culture, TGF-β1 can direct the differentiation of mesenchymal stem cells into osteoblasts, chondrocytes, or adipocytes. In organoid culture systems, it regulates extracellular matrix (ECM) formation and influences tissue organization. Additionally, TGF-β1 exhibits immunomodulatory effects by suppressing the activation of T cells, B cells, NK cells, and macrophages, thereby reducing inflammatory responses. In co-culture systems—such as those involving immune and tumor cells—TGF-β1 can help mimic an immune-suppressive microenvironment.

Specifications

Product Details
Cat. No.	Y04201N
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.1ng/mL.
Accession	P01137
Alternative Names	Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated Peptide; LAP; TGFB1; TGFB
Species	Human
Expression System	CHO
Mol. Weight	12.8 kDa
Expression Region	Ala279-Ser390
Tag Info	Tag free
Form	Lyophilized
Buffer	0.085% TFA, 30% ACN, 5% Mannitol, pH 2.5.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in 4mM HCL.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Regulates hematopoietic stem cell quiescence and lineage balance.

Immunology

Strong immunosuppressive factor, modulating Tregs and inhibiting effector T cells.

Stem Cell Research

Maintains stem cell quiescence and guides differentiation; used in organoid cultures.

Cell Biology

Regulates proliferation, apoptosis, ECM production, and EMT.

Data Figures

Figure 1: Recombinant Human TGF-β1 (Cat. No. Y04201N) SDS-PAGE under reducing (R) & Non-reducing condition. The gel was stained with Y04201.

Measured in a cell inhibition assay using TF-1 cells at IL-4 presence. The ED50 for this effect is ≤0.1 ng/mL. The bioactivity of Y04201N was higher than other competing products.

Figure 2: Measured in a cell inhibition assay using TF-1 cells at IL-4 presence. The ED50 for this effect is ≤0.1 ng/mL. The bioactivity of Y04201N was higher than other competing products.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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