Description

R-Spondin-1 (RSPO1) is a secreted protein belonging to the R-Spondin family and plays a key regulatory role in the Wnt/β-catenin signaling pathway. By binding to Wnt receptors LRP5/6 and transmembrane proteins LGR4/5/6, RSPO1 inhibits negative regulators of the Wnt pathway and reduces the degradation of Wnt receptors, thereby significantly enhancing Wnt/β-catenin signaling activity. Through potentiation of Wnt signaling, RSPO1 promotes cell proliferation, stemness maintenance, and tissue regeneration, making it particularly valuable in stem cell culture. In 3D organoid culture systems, RSPO1—when combined with factors such as EGF and Noggin—significantly improves organoid formation efficiency and long-term viability. In stem cell cultures, its use together with Wnt3a supports self-renewal and suppresses differentiation. In summary, R-Spondin-1 is a critical factor in stem cell and organoid culture systems. By modulating the Wnt signaling pathway, it facilitates cell proliferation and maintenance of stemness, and is widely applied in regenerative medicine and disease modeling research.

Specifications

Product Details
Cat. No.	Y03501
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured by its ability to induce Topflash reporter bioactivity in CHO cells in the presence of 50 ng/mL wnt3a. The expected ED50 is <1.0 μg/mL.
Accssion	Q2MKA7
Alternative Names	RSPO1; R-spondin1; RP11-566C13.1; CRISTIN3; FLJ40906; RSPO Rspo1; R-spondin; Rspondin; RP23-325M14.2; Roof
Species	Human
Expression System	CHO
Mol. Weight	26.7 kDa
Expression Region	Ser21-Ala263
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Stem Cell Research

Potent activator of Wnt signaling to expand intestinal and organoid stem cells.

Cell Biology

Enhances Wnt/尾-catenin-mediated proliferation and stem cell maintenance.

Data Figures

Figure 1: Recombinant Human R-Spondin-1 (Cat. No. Y03501) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y03501 SDS-PAGE.

Measured by its ability to induce Topflash reporter bioactivity in CHO cells in the presence of 50 ng/mL wnt3a. The expected ED50 is＜1.0 μg/mL. The bioactivity of Recombinant Human R-Spondin-1(Cat. No. Y03501) was higher than other competing products.

Figure 2: Measured by its ability to induce Topflash reporter bioactivity in CHO cells in the presence of 50 ng/mL wnt3a. The expected ED50 is＜1.0 μg/mL. The bioactivity of Recombinant Human R-Spondin-1(Cat. No. Y03501) was higher than other competing products.

Figure 3: Highly consistent bioactivity between different production batches of Recombinant Human R-Spondin-1 (Cat. No. Y03501).

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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