{"product_id":"recombinant-human-r-spondin-1","title":"Recombinant Human R-Spondin-1","description":"\u003cstyle\u003e.biofargo-table-wrapper {overflow-x: auto;margin: 20px 0;-webkit-overflow-scrolling: touch;}.biofargo-spec-table {width: 100%;border-collapse: collapse;border: 1px solid #dee2e6;min-width: 600px;background: #fff;}.biofargo-spec-table th,.biofargo-spec-table td {border: 1px solid #dee2e6;padding: 12px;vertical-align: middle;white-space: normal;word-break: break-word;}@media (max-width: 600px) {.biofargo-table-wrapper {border: 1px solid #eee;border-radius: 8px;padding: 6px;background: #fff;}.biofargo-spec-table {min-width: 720px;}}\u003c\/style\u003e\u003cdiv style=\"font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1000px; margin: 0 auto; border: 1px solid #e1e8ed; padding: 30px; border-radius: 10px; background-color: #ffffff;\"\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 0; font-size: 22px;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #fcfcfc; padding: 15px; border-radius: 6px; border: 1px solid #f0f0f0;\"\u003e\u003cp style=\"margin: 0; text-align: justify;\"\u003eR-Spondin-1 (RSPO1) is a secreted protein belonging to the R-Spondin family and plays a key regulatory role in the Wnt\/β-catenin signaling pathway. By binding to Wnt receptors LRP5\/6 and transmembrane proteins LGR4\/5\/6, RSPO1 inhibits negative regulators of the Wnt pathway and reduces the degradation of Wnt receptors, thereby significantly enhancing Wnt\/β-catenin signaling activity. Through potentiation of Wnt signaling, RSPO1 promotes cell proliferation, stemness maintenance, and tissue regeneration, making it particularly valuable in stem cell culture. In 3D organoid culture systems, RSPO1—when combined with factors such as EGF and Noggin—significantly improves organoid formation efficiency and long-term viability. In stem cell cultures, its use together with Wnt3a supports self-renewal and suppresses differentiation. In summary, R-Spondin-1 is a critical factor in stem cell and organoid culture systems. By modulating the Wnt signaling pathway, it facilitates cell proliferation and maintenance of stemness, and is widely applied in regenerative medicine and disease modeling research.\u003c\/p\u003e\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrapper\"\u003e\u003ctable class=\"biofargo-spec-table\"\u003e\n\u003cthead\u003e\u003ctr style=\"background-color: #0056b3; color: white;\"\u003e\u003cth style=\"padding: 12px; text-align: left;\" colspan=\"2\"\u003eProduct Details\u003c\/th\u003e\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eCat. No.\u003c\/td\u003e\n\u003ctd\u003eY03501\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003ePurity\u003c\/td\u003e\n\u003ctd\u003e≥95%, by SDS-PAGE (under reducing (R) \u0026amp; Non-reducing conditions, visualized by Coomassie staining).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eEndotoxin\u003c\/td\u003e\n\u003ctd\u003e≤10 EU\/mg by the LAL method.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eBioactivity\u003c\/td\u003e\n\u003ctd\u003eMeasured by its ability to induce Topflash reporter bioactivity in CHO cells in the presence of 50 ng\/mL Wnt3a. The expected ED50 is \u0026lt;1.0 μg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eAccession\u003c\/td\u003e\n\u003ctd\u003eQ2MKA7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eAlternative Names\u003c\/td\u003e\n\u003ctd\u003eRSPO1; R-spondin1; RP11-566C13.1; CRISTIN3; FLJ40906; RSPO Rspo1; R-spondin; Rspondin; RP23-325M14.2; Roof\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eSpecies\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eExpression System\u003c\/td\u003e\n\u003ctd\u003eCHO\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eMol. Weight\u003c\/td\u003e\n\u003ctd\u003e26.7 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eExpression Region\u003c\/td\u003e\n\u003ctd\u003eSer21-Ala263\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eTag Info\u003c\/td\u003e\n\u003ctd\u003eTag free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eFormulation\u003c\/td\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eBuffer\u003c\/td\u003e\n\u003ctd\u003ePBS, 5% Mannitol and 0.01% Tween 80, pH7.4.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eSize\u003c\/td\u003e\n\u003ctd\u003e10 μg \/ 50 μg \/ 1 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eLead Time\u003c\/td\u003e\n\u003ctd\u003eProducts are in stock, but custom products may require further consultation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eReconstitution\u003c\/td\u003e\n\u003ctd\u003eIt is recommended to redissolve in sterile deionized water.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eThe product is shipped with blue ice.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\" width=\"26%\"\u003eStorage \u0026amp; Stability\u003c\/td\u003e\n\u003ctd\u003e36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eHigh Purity:\u003c\/strong\u003e This product undergoes stringent purification processes, achieving a purity level of over 95%, ensuring the reliability and reproducibility of experimental results.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eHigh Biological Activity:\u003c\/strong\u003e Verified through multiple activity assays, this product effectively induces the expected biological responses in target cells.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eBatch Consistency:\u003c\/strong\u003e Manufactured under strict production and quality control protocols to ensure highconsistency between batches.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eAnimal-Free:\u003c\/strong\u003e Produced without the use of animal-derived components, meeting animal-free requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eApplications\u003c\/h2\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 20px;\"\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eStem Cell Research\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003ePotent activator of Wnt signaling to expand intestinal and organoid stem cells.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eCell Biology\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eEnhances Wnt\/β-catenin-mediated proliferation and stem cell maintenance. and stem cell maintenance.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eData Figures\u003c\/h2\u003e\n\u003cdiv style=\"text-align: center; margin-top: 20px; border: 1px solid #eee; padding: 15px; border-radius: 8px;\"\u003e\n\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"Recombinant Human R-Spondin-1 (Cat. No. Y03501) SDS-PAGE under reducing (R) \u0026amp; Non-reducing conditions. The gel was stained with Y03501 SDS-PAGE.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_36651fda-ed0c-4484-87ba-ac78d7b9cbb2.jpg?v=1779779365\"\u003e\u003cp style=\"font-size: 13px; color: #888; margin-top: 10px;\"\u003eFigure 1: Recombinant Human R-Spondin-1 (Cat. No. Y03501) SDS-PAGE under reducing (R) \u0026amp; Non-reducing conditions. The gel was stained with Y03501 SDS-PAGE.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"text-align: center; margin-top: 20px; border: 1px solid #eee; padding: 15px; border-radius: 8px;\"\u003e\n\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"Measured by its ability to induce Topflash reporter bioactivity in CHO cells in the presence of 50 ng\/mL Wnt3a. The expected ED50 is＜1.0 μg\/mL. The bioactivity of Recombinant Human R-Spondin-1(Cat. No. Y03501) was higher than other competing products.\" src=\"https:\/\/files.biofargo.com\/eastmabbio\/product-detail-images\/Y03501_2.jpg\"\u003e\u003cp style=\"font-size: 13px; color: #888; margin-top: 10px;\"\u003eFigure 2: Measured by its ability to induce Topflash reporter bioactivity in CHO cells in the presence of 50 ng\/mL Wnt3a. The expected ED50 is＜1.0 μg\/mL. The bioactivity of Recombinant Human R-Spondin-1(Cat. No. Y03501) was higher than other competing products.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"text-align: center; margin-top: 20px; border: 1px solid #eee; padding: 15px; border-radius: 8px;\"\u003e\n\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"Highly consistent bioactivity between different production batches of Recombinant Human R-Spondin-1 (Cat. No. Y03501).\" src=\"https:\/\/files.biofargo.com\/eastmabbio\/product-detail-images\/Y03501_3.jpg\"\u003e\u003cp style=\"font-size: 13px; color: #888; margin-top: 10px;\"\u003eFigure 3: Highly consistent bioactivity between different production batches of Recombinant Human R-Spondin-1 (Cat. No. Y03501).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eInstructions for Use\u003c\/h2\u003e\n\u003cul style=\"padding-left: 20px;\"\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eDissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003ePrepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eDo not mix with other similar products to avoid cross-reactivity and potential interference.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eCautions\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #fff5f5;  padding: 15px; color: #852d2d; font-size: 14px;\"\u003e\n\u003cp style=\"margin: 0 0 8px 0;\"\u003e\u003cstrong\u003e• Research Use Only:\u003c\/strong\u003e This product is not suitable for clinical diagnostics or therapeutic applications.\u003c\/p\u003e\n\u003cp style=\"margin: 0 0 8px 0;\"\u003e\u003cstrong\u003e• Preservation:\u003c\/strong\u003e Handle according to instructions to preserve its activity and stability.\u003c\/p\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cstrong\u003e• Support:\u003c\/strong\u003e Contact our technical support team if you encounter any issues or questions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"display: flex; gap: 15px; margin-top: 10px; flex-wrap: wrap;\"\u003e\n\u003ca style=\"display: inline-block; padding: 10px 20px; border: 2px solid #0056b3; color: #0056b3; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/files.biofargo.com\/eastmabbio\/coa\/20251021%20-Y03501-%20Recombinant%20Human%20R-Spondin%201-10%CE%BCg%20-%20%E5%85%A8%E8%8B%B1%E7%89%88.pdf\" target=\"_blank\"\u003e📄 Download COA\u003c\/a\u003e \u003c\/div\u003e\n\u003c\/div\u003e","brand":"EastMabBio","offers":[{"title":"10ug","offer_id":52404307689653,"sku":"Y03501-10ug","price":71.0,"currency_code":"USD","in_stock":true},{"title":"50ug","offer_id":52284505129141,"sku":"Y03501-50ug","price":246.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":52284505161909,"sku":"Y03501-1mg","price":2442.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Y03501_Recombinant_Human_R-Spondin1.jpg?v=1771998082","url":"https:\/\/biofargo.com\/products\/recombinant-human-r-spondin-1","provider":"Biofargo","version":"1.0","type":"link"}