Description

PDGF-BB (Platelet-Derived Growth Factor-BB) is an important growth factor belonging to the platelet-derived growth factor (PDGF) family. It consists of two identical B chains linked by disulfide bonds to form a homodimer and is one of the most biologically potent isoforms in the PDGF family. PDGF-BB activates PDGF receptors (PDGFR-α and PDGFR-β) on the cell membrane, triggering downstream signaling pathways that promote the proliferation of various cell types—particularly fibroblasts, smooth muscle cells, glial cells, and mesenchymal stem cells. Additionally, in processes such as wound healing, angiogenesis, and tissue regeneration, PDGF-BB acts as a chemoattractant, guiding cells such as fibroblasts and pericytes to sites of injury to facilitate tissue repair. It also contributes to the stabilization of newly formed blood vessels and works synergistically with VEGF to promote vascular formation.

Specifications

Product Details
Cat. No.	Y03411
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	The ED50 for this effect is ≤10 ng/mL.
Accssion	P01127
Alternative Names	PDGFBB; PDGF-BB
Species	Human
Expression System	CHO
Mol. Weight	12.2 kDa
Expression Region	Ser82-Thr190
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Stem Cell Research

Promotes MSC proliferation and vascular support in organoid/engineered tissues.

Cell Biology

Regulates proliferation, migration, and survival of mesenchymal and vascular cells.

Data Figures

Figure 1: Recombinant Human PDGF-BB (Cat. No. Y03411) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y03411 SDS-PAGE.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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