Description

PDGF-AB (Platelet-Derived Growth Factor-AB) is an important dimeric protein growth factor belonging to the PDGF family, formed by one A chain and one B chain linked via disulfide bonds. It is primarily secreted by platelets, endothelial cells, macrophages, and other cell types. In mesenchymal stem cell (MSC) culture, PDGF-AB promotes proliferation and helps maintain multipotent differentiation potential. It also inhibits apoptotic pathways and enhances cell survival, which is particularly critical under serum-free or low-serum culture conditions. PDGF-AB plays a key role in tissue repair, embryonic development, and cell proliferation.

Specifications

Product Details
Cat. No.	Y12101
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measure in a cell proliferation assay using NIH3T3 cells. The ED50 is ≤1.0 ng/mL.
Alternative Names	PDGFAB; PDGF-AB
Species	Human
Expression System	CHO
Mol. Weight	14.3 kDa & 12.3 kDa
Expression Region	Ser87-Thr211 & Ser82-Thr190
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Stem Cell Research

Enhances MSC proliferation and angiogenic support in vitro.

Cell Biology

Stimulates mesenchymal cell proliferation and migration.

Data Figures

Figure 1: Measured in a cell proliferation assay using NlH3T3 cells. The ED50 is ≤ 1.0ng/mL.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human PDGF-AB