Description

Oncostatin M (OSM) belongs to the interleukin-6 cytokine family.

OSM is a glycoprotein secreted by activated monocytes, macrophages, T lymphocytes, and dendritic cells.

OSM was first discovered as a factor that inhibits melanoma cell growth.

OSM inhibits liver cancer cell proliferation by activating STAT3 signaling and upregulating oncogene c-Myc expression, while also accelerating cell senescence.

In addition, OSM participates in liver cell proliferation and tissue remodeling during liver regeneration.

OSM can also inhibit the adipogenic differentiation of human bone marrow mesenchymal stem cells and stimulate their osteogenic differentiation.

Specifications

Parameter	Specification
Expression Host	HEK293 cells
Synonyms	Oncostatin M; OSM; MGC20461
Protein Sequence	Amino acid sequence: Ala26–Arg221 of human OSM (UniProt ID: P13725)
Molecular Weight	Predicted molecular weight is 23.24 kDa
Purity	>95%, analyzed by SDS-PAGE and HPLC
Endotoxin	<1 EU/μg
Purification Method	Chromatographic purification
Appearance	White lyophilized powder
Activity	Refer to COA for details

Features

✔ Recombinant Human Oncostatin M Protein
Human OSM corresponding to Ala26–Arg221 produced in HEK293 cells with a predicted molecular weight of 23.24 kDa.
✔ High-Purity Chromatographic Preparation
Purity >95% confirmed by SDS-PAGE and HPLC following chromatographic purification.
✔ Controlled Endotoxin Level
Endotoxin content <1 EU/μg, supporting reliable performance in sensitive in vitro cell culture experiments.
✔ Defined Lyophilized Protein Format
Supplied as a white lyophilized powder with verified protein identity and consistent biological activity.

Applications

Liver Regeneration & Hepatocyte Biology

Participates in hepatocyte proliferation and tissue remodeling processes during liver regeneration studies.

Cancer Biology Research

Used to investigate STAT3 signaling activation and c-Myc regulation involved in liver cancer cell proliferation and senescence mechanisms.

Mesenchymal Stem Cell Differentiation

Inhibits adipogenic differentiation of human bone marrow mesenchymal stem cells while promoting osteogenic differentiation.

Stem Cell Culture Systems

Applicable for stem cell culture models including MSC and iPSC research environments.

Storage

Store below -20°C

Instructions

Centrifuge before reopening the tube. Dissolve the lyophilized protein in distilled water. It is recommended to reconstitute the concentration to greater than 100 μg /mL.

After reconstitution, the protein can be stored at -80℃ for 3-6 months and at 2-8℃ for 2-7 days. For optimal storage, aliquot the protein and minimize freeze-thaw cycles.

Precautions

This product is only suitable for in vitro cell culture and cannot be used directly in clinical treatment.

Documents

SDS User Manual

Recombinant Human OSM Protein