Description

M-CSF (Macrophage Colony-Stimulating Factor) is an important glycoprotein cytokine that exists as a disulfide-linked homodimer. It plays a key role in regulating the proliferation, differentiation, and survival of the monocyte-macrophage lineage. M-CSF promotes the proliferation of monocytes/macrophages, supports the differentiation of hematopoietic stem cells into monocytes and their subsequent development into macrophages, and enhances macrophage survival through anti-apoptotic signaling (e.g., upregulation of Bcl-2). Additionally, it modulates functional polarization and influences macrophage phenotype.

Specifications

Product Details
Cat. No.	Y06721H
Purity	≥95%, by SDS-PAGE (under reducing (R)& Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤100 EU/mg by the LAL method.
Bioactivity	The activity is determined by the dose-dependent proliferation of murine NFS-60 cells is＜0.5 ng/mL.
Accession	P09603.3
Species	Human
Expression System	E. coli
Mol. Weight	18.4 kDa
Expression Region	Glu33-Ser190
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, 5% Trehalose and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Promotes proliferation and differentiation of monocyte/macrophage progenitors.

Immunology

Regulates macrophage survival and function.

Stem Cell Research

Used to generate macrophages from HSCs or iPSCs.

Cell Biology

Controls macrophage proliferation, differentiation, and survival.

Data Figures

Figure 1: Measured in a cell proliferation assay using M-NFS-60 cells

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human M-CSF