Description

M-CSF is a disulfide-linked homodimeric glycoprotein and core member of the colony-stimulating factor family. By specifically binding the c-Fms receptor (CSF-1R/CD115), it induces receptor dimerization and internalization and activates PI3K-AKT/mTOR and Ras-MAPK signaling to drive hematopoietic stem cell differentiation toward monocyte/macrophage lineages while promoting osteoclast precursor proliferation and bone remodeling.

This protein exists as two bioactive isoforms: glycosylated and chondroitin sulfate-modified proteoglycan variants—the latter extends local bioactivity via extracellular matrix anchoring.

Immunologically, it mediates endothelial progenitor mobilization and neovascularization through macrophage-derived VEGF secretion while upregulating proinflammatory chemokines to potentiate innate immunity.

For cell therapy applications, it serves as a critical component in:

ex vivo macrophage differentiation systems, synergizing with GM-CSF to induce M1-polarized anti-tumor macrophages for CAR-M therapies with enhanced tumor infiltration and phagocytosis;
3D bioscaffold cultures where spatiotemporal release kinetics engineer functional vascular networks to improve survival of tissue-engineered grafts.

Specifications

Specification	Acceptance Criteria	TL-514-0010	TL-514-0050
Appearance	Freeze-dried preparation, the form should be white loose body	Compliance	Compliance
Identity	The electrophoretic bands should be consistent with the molecular weight of the product	Compliance	Compliance
Visible Particle	Should be no visible particle	Compliance	Compliance
Moisture	≤4.0%	0.9%	0.6%
pH	6.5~7.5	7.2	7.2
Purity	>90.0%	Compliance	Compliance
Endotoxin	<0.1EU/μg	<0.1EU/μg	<0.1EU/μg
Mycoplasma	Should be negative	Negative	Negative
Sterility	No growth	No growth	No growth
Biological Activity	Cell proliferation assay was performed with M-NFS-60 cells, and the activity ≥1×10⁷ IU/mg	2.50×10⁷ IU/mg	3.88×10⁷ IU/mg

Features

✔ Native Dimeric M-CSF Activity
Disulfide-linked homodimer preserves authentic CSF-1R (c-Fms) activation and downstream signaling.
✔ High Biological Potency
Verified by M-NFS-60 cell proliferation assay with specific activity ≥1×10⁷ IU/mg (lot values up to 3.88×10⁷ IU/mg).
✔ Low Endotoxin, Stringent QC
90% purity with endotoxin <0.1 EU/µg; sterility and mycoplasma tested for sensitive cell culture use.
✔ Consistent Lot Performance
Tight control of moisture and pH supports reproducible differentiation outcomes.

Applications

Macrophage Differentiation Systems

Drives monocyte/macrophage lineage commitment for in vitro immune cell studies.

CAR-M & Tumor Immunology Research

Used alone or with GM-CSF to support macrophage activation and functional polarization.

3D Tissue Engineering & Vascularization

Facilitates macrophage-mediated angiogenic support in scaffold-based cultures.

Bone & Osteoclast Research

Supports studies on osteoclast precursor proliferation and bone remodeling pathways.

Stability & Storage

Lyophilized powder: Stable for 12 months at −80 °C or 6 months at −20 °C when stored in the original sealed container under desiccant.
Reconstitution: Dissolve in sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4), maintaining a final concentration ≥100 µg/mL.
Handling: After reconstitution, aliquot and avoid repeated freeze–thaw cycles.

Cautions

• Research Use Only: This product is not intended for diagnostic or therapeutic use.

• Handling: Prepare solutions using sterile technique. Avoid microbial contamination to maintain activity.

• Storage: Follow recommended storage conditions. Repeated freeze–thaw cycles may reduce performance.

Documents

📄 TL-514 Data Sheet 📄 TL-514 SDS 📄 TL-514-0010 COA 📄 TL-514-0050 COA

Recombinant Human M-CSF Protein