Description

Recombinant Human Long Arg3 Insulin-like Growth Factor 1 (LR3 IGF-1) is a small peptide growth factor that plays a critical role in cell proliferation, differentiation, and survival. It is widely involved in growth and development processes and mediates many of the biological effects of growth hormone. IGF-1 binds to the IGF-1 receptor (IGF1R), activating downstream signaling pathways such as PI3K/AKT and MAPK, which regulate cell growth and metabolism. This recombinant LR3 IGF-1 protein is expressed in CHO cells and corresponds to the engineered human LR3 IGF-1 sequence, ensuring proper folding and biological functionality. With high purity (≥95%) and strong bioactivity (ED50 ≤1.5 ng/mL in MCF-7 cell proliferation assay), this product is ideal for research in oncology, cell biology, and metabolic regulation. The protein is supplied in a tag-free, lyophilized form for convenient storage and reconstitution.

Specifications

Product Details
Cat. No.	Y03101
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is ≤1.5 ng/mL.
Accession	P05019
Species	Human
Expression System	CHO
Mol. Weight	9 kDa
Expression Region	MFPAMPLSSLFVN+Gly49-Ala118(E51R)
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm filtered solution in Acetic Acid.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.
✔ High Biological Activity: Demonstrates potent activity with an ED50 ≤1.5 ng/mL in MCF-7 cell proliferation assay, ensuring strong biological performance.
✔ Mammalian Expression System: Expressed in CHO cells to ensure correct folding and native-like post-translational modifications.
✔ Flexible Application Range: Suitable for a wide range of studies including cancer research, cell proliferation, and signaling pathway analysis.

Applications

Cancer Research

Widely used in tumor biology studies, especially in breast cancer models such as MCF-7 cell proliferation assays.

Metabolism Research

Involved in glucose metabolism, growth regulation, and hormone signaling studies.

Cell Biology

Promotes cell proliferation, survival, and differentiation via IGF-1 receptor-mediated signaling pathways.

Data Figures

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is ≤1.5 ng/mL. The bioactivity of Y03101 is higher than other competing products.

Figure 1: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is ≤1.5 ng/mL. The bioactivity of Y03101 is higher than other competing products.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Recombinant Human LR3 IGF-1