Description

LIF (Leukemia Inhibitory Factor) is a pleiotropic cytokine belonging to the IL-6 family. As a secreted glycoprotein, it activates downstream signaling pathways by binding to the LIFR/gp130 receptor complex and plays a critical role in cell culture, particularly in stem cell maintenance. Its primary function is to sustain pluripotency in mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). In other stem cell contexts, LIF promotes the survival and proliferation of neural stem cells and, in combination with factors such as SCF and IL-3, helps maintain the stemness of hematopoietic stem cells.

Specifications

Product Details
Cat. No.	Y03001
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/mL.
Accession	P15018
Alternative Names	Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor; D Factor; Melanoma-Derived LPL Inhibitor; MLPLI; Emfilermin; LIF; HILDA
Species	Human
Expression System	CHO
Mol. Weight	19.7 kDa
Expression Region	Ser23-Phe202
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Maintains hematopoietic progenitor survival in vitro.

Immunology

Supports certain immune cell subsets (e.g., Tregs) indirectly.

Stem Cell Research

Widely used to maintain pluripotency of ESCs and support stem cell survival.

Cell Biology

Regulates cell survival, proliferation, and differentiation via JAK/STAT3 signaling.

Data Figures

Figure 1: Recombinant Human LIF (Cat. No. Y03001) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y03001 SDS-PAGE.

Figure 2: Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/mL.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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