Description

Recombinant Human Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine that plays a crucial role in cell differentiation, proliferation, and survival. It belongs to the IL-6 cytokine family and signals through the LIF receptor (LIFR) and gp130 complex, activating downstream pathways such as JAK/STAT3. LIF is widely known for its essential role in maintaining the pluripotency of embryonic stem cells and regulating hematopoiesis and immune responses.

This recombinant protein is expressed in E. coli and corresponds to the mature region (Ser23–Phe202) of human LIF, retaining full biological activity. With high purity (≥95%) and strong bioactivity (ED50 ＜0.5 ng/mL, measured by inhibition of M1 cell proliferation), this product is suitable for stem cell culture, immunology, and cancer research. The protein is supplied in a tag-free, lyophilized form for convenient storage and reconstitution.

Specifications

Product Details
Cat. No.	Y03001H
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically ＜0.5 ng/mL.
Accession	NP_001244064.1
Alternative Names	Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor; D Factor; Melanoma-Derived LPL Inhibitor; MLPLI; Emfilermin; LIF; HILDA
Species	Human
Expression System	E. coli
Mol. Weight	19.7 kDa
Expression Region	Ser23-F202
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, 5% Trehalose and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.
✔ High Biological Activity: Exhibits potent activity with ED50 ＜0.5 ng/mL in M1 cell proliferation inhibition assay, ensuring consistent performance.
✔ Optimized Expression System: Expressed in E. coli, enabling efficient production and suitability for routine laboratory applications.
✔ Convenient and Reliable: Supplied in a tag-free, lyophilized form for easy reconstitution, stable storage, and compatibility with various experimental workflows.

Applications

Stem Cell Research

Maintains pluripotency and self-renewal of embryonic stem cells via activation of the JAK/STAT3 pathway.

Immunology

Regulates immune responses and cytokine signaling within the IL-6 family network.

Cancer Research

Involved in studies of cell differentiation and proliferation, particularly in leukemia and tumor biology models.

Data Figures

Measured in a cell inhibitionn assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2 ng/mL. The bioactivity of Y07901 was higher than other competing products.

Figure 1: Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically ＜0.5 ng/mL.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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Recombinant Human LIF