Description

LN521E8 (Laminin-511 E8 Fragment) is an E8 fragment of laminin-521 that provides robust support for stem cell self-renewal and survival. It is commonly used for iPSC/ESC maintenance, single-cell passaging, and clinical-grade stem cell culture systems.

Specifications

Product Details
Code	Y13342HPurity≥95%, by SDS-PAGE (under reducing (R)& Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured by the ability of the immobilized protein to support the adhesion of NIH3T3 cells. The ED50 for this effect is ≤10 ng/mL.
Accession	O15230&P55268&P11047
Species	Human
Expression System	HEK293
Mol. Weight	141.0 KDa
Expression Region	Ala2534-Ala3322&Leu1573-Gln1798&Asn1364-Pro1609
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Supports HSC adhesion in vitro. on.

Stem Cell Research

Maintains ESC/iPSC pluripotency and improves survival in culture.

Cell Biology

Provides basement membrane cues, promotes adhesion and proliferati

Data Figures

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human Laminin521 E8