Description

LN511E8 (Laminin-511 E8 Fragment) is a recombinant laminin fragment derived from laminin-511 that supports strong integrin-mediated cell adhesion. It is widely used as a defined matrix for human pluripotent stem cell attachment and expansion under xeno-free conditions.

Specifications

Product Details
Code	Y13242H
Purity	≥95%, by SDS-PAGE (under reducing (R)& Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured by the ability of the immobilized protein to support the adhesion of NIH3T3 cells. The ED50 for this effect is ≤10 ng/mL.
Accession	O15230&P07942&P11047
Species	Human
Expression System	HEK293
Mol. Weight	141.6 KDa
Expression Region	Ala2534-Ala3323&Leu1561-Leu1786&Asn1364-Pro1609
Tag Info	His Tag
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Supports HSC adhesion in vitro.

Stem Cell Research

Maintains pluripotency and adhesion of ESCs/iPSCs in defined culture systems.

Cell Biology

Provides basement membrane cues, regulates adhesion and survival.

Data Figures

Figure 1: Measured in a cell proliferation assay using Nb2-11 cells.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human Laminin511 E8