Description

IL-7 (Interleukin-7) is a cytokine produced by bone marrow stromal cells, thymic epithelial cells, and other cell types. It belongs to the four-helix bundle cytokine family and exerts its functions primarily by binding to the IL-7 receptor, thereby activating signaling pathways such as JAK-STAT, PI3K-AKT, and MAPK. IL-7 plays an essential role in the immune system, particularly in T cell development, survival, and homeostatic proliferation. In in vitro cultures, IL-7 supports the survival of naïve and memory T cells and helps prevent apoptosis. During early B cell development, it promotes both proliferation and differentiation. In the context of cell therapy—such as CAR-T or TCR-T treatments—IL-7 is often used in combination with other cytokines like IL-15 to enhance T cell expansion and maintain stem-like properties. Furthermore, IL-7 can improve the yield and activity of tumor-infiltrating lymphocytes (TILs) or virus-specific T cells during culture.

Specifications

Product Details
Cat. No.	Y02401
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using NALM-6 human pre-B cells. The ED50 for this effect is ≤30 ng/mL.
Accession	P13232
Alternative Names	Interleukin-7; IL-7; IL7
Species	Human
Expression System	CHO
Mol. Weight	17.3 kDa
Expression Region	Asp26-His177
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at 2°C to 8°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Supports lymphoid cell development and survival.

Immunology

Maintains T cell homeostasis and immune memory.

Cell Biology

Regulates cell survival and anti-apoptotic signaling.

Data Figures

Figure 1: Recombinant Human IL-7 (Cat. No. Y02401) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y02401 SDS-PAGE.

Figure 2: Measured in a cell proliferation assay using NALM-6 human pre-B cells. The ED50 for this effect is ≤30 ng/mL.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA