Description

IL-6 (Interleukin-6) is a pleiotropic cytokine secreted by various cell types, including T cells, B cells, macrophages, fibroblasts, and endothelial cells. It plays a critical role in immune regulation, inflammatory responses, hematopoiesis, metabolism, and tissue repair. Signaling is mediated through a complex composed of gp130 and the IL-6 receptor (IL-6R), activating pathways such as JAK/STAT, MAPK, and PI3K. In immune cell cultures—including T cells, B cells, and macrophages—IL-6 promotes Th17 cell differentiation (associated with autoimmunity), suppresses regulatory T cell (Treg) function, and stimulates B cell differentiation into plasma cells, thereby enhancing antibody production. In hematopoietic stem cell cultures, IL-6 acts synergistically with other cytokines (e.g., IL-3 and SCF) to promote the proliferation and differentiation of hematopoietic progenitors, particularly along the megakaryocyte and granulocyte lineages.

Specifications

Product Details
Cat. No.	Y02301
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using M-NSF-60 cells. The ED50 for this effect is 0.2-1 ng/mL.
Accession	P05231
Alternative Names	Interleukin-6; IL-6; B-Cell Stimulatory Factor 2; BSF-2; CTL Differentiation Factor; CDF; Hybridoma Growth Factor; Interferon Beta-2; IFN-Beta-2; IL6; IFNB2
Species	Human
Expression System	CHO
Mol. Weight	20.8 kDa
Expression Region	Val30-Met212
Tag Info	Tag free
Form	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at 2°C to 8°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Modulates hematopoietic response under inflammation and B cell differentiation.

Immunology

Links innate and adaptive immunity; involved in inflammatory and immune dysregulation.

Stem Cell Research

Used to mimic inflammatory microenvironments, affecting stem cell fate.

Cell Biology

Regulates proliferation, survival, and stress response signaling.

Data Figures

Figure 1: Recombinant Human IL-6 (Cat. No. Y02301) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y02301 SDS-PAGE.

Figure 2: Measured in a cell proliferation assay using M-NFS-60 cells. The ED50 for this effect is 0.2-1 ng/mL.

Figure 3: Highly consistent bioactivity between different production batches of Recombinant Human IL-6. (Cat. No. Y02301).

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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