Product Information

Protein sequence	A DNA sequence encoding the human IL-6（NP_000591.1：V30-M212）was expressed with a His-tag at the C-terminus
Expression Host	HEK293 cells
QC Testing Purity	> 90 % as determined by SDS-PAGE
Activity	Determined by the dose-dependent stimulation of the proliferation of TF-1 cells. The expected ED₅₀ for this effect is ≤ 1 ng/mL
Endotoxin Level	< 0.1 EU per μg of the protein as determined by the LAL method
Molecular Mass	The Recombinant Human IL-6 Protein predicts a molecular mass of 21.6 kD
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization
Stability & Storage	24 months at 2 °C to 8 °C in lyophilized state. 6 months at -20 °C under sterile conditions after reconstitution. 12 months at -80 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100 µg/mL. Avoid repeated freeze-thaw cycles.

Background

Interleukin 6 (IL-6) is produced by fibroblasts, monocytes, macrophages, T lymphocytes, B lymphocytes, epithelial cells, keratinocytes and various tumor cells. IL-1, TNF-α, PDGF, viral infection, double stranded RNA, and cAMP can all induce normal cells to produce IL-6. IL-6 can stimulate the proliferation, differentiation of immune responses and improve immune cell functions. IL-6R is widely expressed on the surface of activated B cells, quiescent T cells, NK cells, myeloma cells, hepatocytes, and myeloid leukemia cells. The main role of IL-6 is to promote the proliferation and differentiation of B cells and the secretion of antibodies. It also has extensive effects on liver cells, T cells, neural tissue, and hematopoietic system. IL-6 also has anti-tumor effects and can directly or indirectly enhance the tumoricidal activity of NK cells and CTL.

References

Ferguson-Smith AC, Chen YF, Newman MS, May LT, Sehgal PB, Ruddle FH (1988)Regional localization of the interferon-beta 2/B-cell stimulatory factor 2/hepatocyte stimulating factor gene to human chromosome 7p15-p21. Genomics 2 (3): 203–8. doi:10.1016/0888-7543(88)90003-1. PMID 3294161.
Van der Poll T, Keogh CV, Guirao X, Buurman WA, Kopf M, Lowry SF (1997)Interleukin-6 gene-deficient mice show impaired defense against pneumococcal pneumonia. The Journal of Infectious Diseases 176 (2): 439–44. doi:10.1086/514062. PMID 9237710.
Banks WA, Kastin AJ, Gutierrez EG (1994)Penetration of interleukin-6 across the murine blood-brain barrier. Neuroscience Letters 179 (1-2): 53–6. doi:10.1016/0304-3940(94)90933-4. PMID 7845624.

Document

GMP-TL512

GMP-TL512_SDS

Recombinant Human IL-6 Protein