Description

The recombinant human IL-6- protein is produced in HEK-293 cells via transient transfection, featuring a C-terminal polyhistidine (His) tag for nickel-affinity purification.

As a pleiotropic α-helical cytokine, IL-6 activates JAK-STAT and MAPK signaling pathways by binding to the IL-6Rα/gp130 heterodimeric receptor, regulating immune responses, hematopoiesis, and cellular differentiation.

In cell and gene therapy applications, it critically enables: ex vivo expansion of hematopoietic stem cells (HSCs) and T cells, enhancement of effector functions in NK and CAR-T cells, directed differentiation of induced pluripotent stem cells (iPSCs) toward hematopoietic lineages, and synergistic construction of vascularized tissue-engineered microenvironments.

The native bioactive conformation is preserved, while the His-tag design ensures precise purification without Fc-mediated off-target effects, making it particularly suitable for regenerative medicine and cell therapy systems requiring spatiotemporal control of cytokine concentrations.

Specifications

Cytokines Details
Synonyms	IL6, Interleukin-6, BSF2, HSF, IFNB2
Accession	UniProt P05231
Expressed Region	Val30–Met212
Tag	C-terminal 6×His-tag
Expression System	HEK293 cells
Molecular Weight	21.6 kDa
Purity	> 95% as determined by SDS-PAGE
Endotoxin	< 0.1 EU per 1 μg of protein (LAL method)
Biological Activity	Measured by TF-1 cell proliferation assay, corresponding to a specific activity of ≥ 5 × 10⁷ IU/mg
Formulation	Lyophilized from sterile PBS (pH 7.4), typically supplemented with 6% mannitol as a stabilizing protectant

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%, ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expected biological responses in target cells, suitable for a wide range of research applications.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure high consistency between batches, reducing variability in experimental outcomes.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements and broadening its application scope in research.

Applications

Immunology

Study the role of IL-6 in immune responses, inflammation, and autoimmune diseases, providing insights into potential therapeutic targets.

Oncology

Investigate the effects of IL-6 on tumor cells, tumor microenvironment, and cancer therapy, aiding in the development of novel anti-cancer strategies.

Hematopoiesis

Explore the role of IL-6 in the regulation of blood cell production and its implications for treating hematological disorders.

Cell Biology

Research the impact of IL-6 on cell signaling pathways, apoptosis, and cellular stress responses, contributing to a deeper understanding of cell biology.

Data Figures

Stability & Storage

Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
Reconstitution: Dissolve in sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration≥100µg/mL to prevent adsorption.
Handling: Aliquot to avoid repeated freeze-thaw cycles.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not intended for diagnostic or therapeutic use.

• Handling: Follow recommended storage and handling instructions to maintain product stability.

• Support: Contact technical support if assistance is required.

Documents

📄 GMP-TL512 SDS 📄 GMP-TL512-0050 COA 📄 GMP-TL512-0100 COA 📄 GMP-TL512 Data Sheet

Recombinant Human IL-6 Protein