Description

IL-5 (Interleukin-5) is a Th2-associated cytokine primarily produced by Th2 cells, type 2 innate lymphoid cells (ILC2s), and mast cells. It signals through the IL-5 receptor complex composed of IL-5Rα and the common βc subunit, activating downstream pathways including JAK-STAT and MAPK. IL-5 plays a central role in eosinophil development, activation, and survival. In in vitro applications, IL-5 is widely used to induce eosinophil differentiation from hematopoietic progenitors and to maintain eosinophil viability and function in culture. It is also commonly applied in immune and inflammation models related to allergy, asthma, and type 2 immune responses.

Specifications

Product Details
Code	Y08701
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is ＜0.1 ng/mL.
Accession	P05113
Species	Human
Expression System	CHO
Mol. Weight	13 kDa
Expression Region	Ile20-Ser134
Tag Info	Tag free
Form	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at-20°C to-80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Specifically promotes eosinophil production and survival.

Immunology

Regulates allergic responses and eosinophil function.

Cell Biology

Supports survival and function of specific immune cells.

Data Figures

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human IL-5