Description

IL-4 (Interleukin-4) is a cytokine primarily secreted by Th2 cells, mast cells, and basophils. It functions by binding to receptor complexes such as IL-4Rα/γc or IL-4Rα/IL-13Rα1, activating the JAK-STAT6 signaling pathway to regulate immune responses, cell proliferation, and differentiation. IL-4 modulates immune cell differentiation and function by promoting Th2 cell polarization and is commonly used in in vitro B cell culture systems. In organoid cultures, IL-4 acts synergistically with IL-13 to drive goblet cell differentiation and modulate the immune microenvironment in intestinal organoids. Additionally, IL-4 can suppress neuronal differentiation and promote the generation of astrocytes.

Specifications

Product Details
Cat. No.	Y02201
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using TF-1 cells, The ED50 for this effect is ≤ 0.2 ng/mL, or responding to a specific bioactivity of ＞1x 107 units/mg.
Accession	P05112
Alternative Names	Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; BSF-1; Binetrakin; Lymphocyte Stimulatory Factor 1; Pitrakinra; IL4
Species	Human
Expression System	CHO
Mol. Weight	15 kDa
Expression Region	His25-Ser153
Tag Info	Tag free
Form	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at 2°C to 8°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Influences B cell maturation and antibody class switching.

Immunology

Drives Th2 differentiation and suppresses Th1 responses.

Stem Cell Research

Mainly used in immune cell polarization models.

Cell Biology

Regulates differentiation programs and phenotypic stability.

Data Figures

Figure 1: Recombinant Human IL-4 (Cat. No. Y02201) SDS-PAGE under reducing (R) condition. The gel was stained with Y02201 SDS-PAGE.

Figure 2: Y02201 stimulates proliferation of TF-1 cells. The ED50 for this effect is ≤0.2 ng/mL.

Figure 3: Highly consistent bioactivity between different production batches of Recombinant Human IL-4 (Cat. No. Y02201)

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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