{"product_id":"recombinant-human-il-4-protein","title":"Recombinant Human IL-4 Protein","description":"\u003cstyle\u003e\n  \/* =========================\n     Biofargo Product Page Base\n  ========================== *\/\n  .biofargo-wrap{\n    font-family:'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color:#333;\n    line-height:1.65;\n    max-width:1000px;\n    margin:0 auto;\n    background:#fff;\n    border:1px solid #e1e8ed;\n    border-radius:12px;\n    padding:28px;\n  }\n  .biofargo-h2{\n    color:#0056b3;\n    border-bottom:2px solid #0056b3;\n    padding-bottom:8px;\n    margin:0 0 14px 0;\n    font-size:22px;\n  }\n  .biofargo-section{ margin-top:28px; }\n  .biofargo-card{\n    background:#fcfcfc;\n    border:1px solid #f0f0f0;\n    border-radius:10px;\n    padding:16px;\n  }\n\n  \/* =========================\n      Table Responsive\n  ========================== *\/\n  .biofargo-table-wrap{\n    overflow-x:auto;\n    -webkit-overflow-scrolling:touch;\n    border:1px solid #eee;\n    border-radius:10px;\n    padding:6px;\n    background:#fff;\n  }\n  table.biofargo-table{\n    width:100%;\n    border-collapse:collapse;\n    font-size:14px;\n    min-width:860px; \/* allow scroll on mobile *\/\n    background:#fff;\n  }\n  table.biofargo-table th,\n  table.biofargo-table td{\n    border:1px solid #ddd;\n    padding:12px;\n    vertical-align:middle;\n    white-space:normal;\n    word-break:break-word;\n  }\n  table.biofargo-table thead th{\n    background:#0056b3;\n    color:#fff;\n    font-weight:700;\n    text-align:left;\n  }\n  table.biofargo-table tbody td:first-child{\n    font-weight:700;\n    background:#f8f9fa;\n  }\n\n  \/* =========================\n      Features (✔ list)\n  ========================== *\/\n  .biofargo-checklist{\n    list-style:none;\n    padding:0;\n    margin:0;\n  }\n  .biofargo-checklist li{\n    position:relative;\n    padding-left:28px;\n    margin:0 0 14px 0;\n  }\n  .biofargo-checklist li:last-child{ margin-bottom:0; }\n  .biofargo-check{\n    position:absolute;\n    left:0;\n    top:0;\n    color:#0056b3;\n    font-weight:800;\n  }\n  .biofargo-checklist strong{\n    display:block;\n    font-size:15px;\n    margin-bottom:4px;\n  }\n  .biofargo-checklist p{\n    margin:0;\n    font-size:14px;\n  }\n\n  \/* =========================\n      Applications (2x2 cards)\n  ========================== *\/\n  .biofargo-grid{\n    display:grid;\n    grid-template-columns:repeat(2, minmax(0, 1fr));\n    gap:16px;\n  }\n  .biofargo-app-card{\n    border:1px solid #e1e8ed;\n    border-radius:12px;\n    padding:14px;\n    background:#fff;\n  }\n  .biofargo-app-card h4{\n    margin:0 0 6px 0;\n    font-size:16px;\n    color:#333;\n  }\n  .biofargo-app-card p{\n    margin:0;\n    font-size:14px;\n  }\n  @media (max-width:640px){\n    .biofargo-wrap{ padding:18px; }\n    .biofargo-grid{ grid-template-columns:1fr; }\n  }\n\n  \/* =========================\n      Warning \/ Cautions\n  ========================== *\/\n  .biofargo-caution{\n    background:#fff5f5;\n    \n    padding:14px;\n    border-radius:10px;\n    color:#852d2d;\n    font-size:14px;\n  }\n  .biofargo-caution p{ margin:0 0 8px 0; }\n  .biofargo-caution p:last-child{ margin:0; }\n\n  \/* =========================\n      Documents (dual CTA)\n  ========================== *\/\n  .biofargo-docs{\n    display:flex;\n    flex-wrap:wrap;\n    gap:12px;\n    margin-top:12px;\n  }\n  .biofargo-btn{\n    display:inline-block;\n    padding:10px 18px;\n    border-radius:8px;\n    font-weight:800;\n    font-size:14px;\n    text-decoration:none;\n  }\n  .biofargo-btn.primary{\n    background:#0056b3;\n    color:#fff;\n    border:2px solid #0056b3;\n  }\n  .biofargo-btn.outline{\n    background:#fff;\n    color:#0056b3;\n    border:2px solid #0056b3;\n  }\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n  \u003c!-- Description --\u003e\n  \u003cdiv class=\"biofargo-section\" style=\"margin-top:0;\"\u003e\n    \u003ch2 class=\"biofargo-h2\"\u003eDescription\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-card\"\u003e\n      \u003cp style=\"margin:0 0 10px 0;\"\u003eAs a master regulator of Th2 immunity, IL-4 activates the JAK1\/3-STAT6 signaling cascade via the IL-4Rα\/γc receptor complex, directing naive CD4+ T-cell differentiation into Th2 effectors secreting IL-5\/IL-13 while mediating B-cell class switching to IgE\/IgG1 and FcεRI upregulation.\u003c\/p\u003e\n      \u003cp style=\"margin:0 0 10px 0;\"\u003eThe recombinant human IL-4-His fusion protein is a high-purity functional cytokine produced in HEK-293 expression systems, featuring a C-terminal hexahistidine tag to facilitate affinity purification and structural integrity.\u003c\/p\u003e\n      \u003cp style=\"margin:0 0 10px 0;\"\u003eIn cell therapy applications, this protein serves as an essential inducer for monocyte-derived immature dendritic cell (imDC) generation through GM-CSF synergy, effectively suppressing CD14 expression and enhancing antigen-capturing capacity to standardize tumor vaccine production.\u003c\/p\u003e\n      \u003cp style=\"margin:0 0 10px 0;\"\u003eIt also modulates CAR-T cell manufacturing by balancing Th1\/Th2 polarization for optimized solid tumor responses and prolongs mast cell survival in vitro to enhance stability of allergen-specific immunotherapies.\u003c\/p\u003e\n      \u003cp style=\"margin:0;\"\u003eThe HEK-293-derived expression ensures authentic glycosylation patterns, while the His-tag maintains native conformation and enables high-resolution chromatographic purification.\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Specifications --\u003e\n  \u003cdiv class=\"biofargo-section\"\u003e\n    \u003ch2 class=\"biofargo-h2\"\u003eSpecifications\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-table-wrap\"\u003e\n      \u003ctable class=\"biofargo-table\"\u003e\n        \u003cthead\u003e\n          \u003ctr\u003e\n            \u003cth style=\"width:30%;\"\u003eSpecification\u003c\/th\u003e\n            \u003cth style=\"width:40%;\"\u003eAcceptance Criteria\u003c\/th\u003e\n            \u003cth style=\"width:30%;\"\u003eResults\u003c\/th\u003e\n          \u003c\/tr\u003e\n        \u003c\/thead\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eAppearance\u003c\/td\u003e\n            \u003ctd\u003eFreeze-dried preparation, the form should be white loose body\u003c\/td\u003e\n            \u003ctd\u003eCompliance\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eIdentity\u003c\/td\u003e\n            \u003ctd\u003eThe electrophoretic bands should be consistent with the molecular weight of the product\u003c\/td\u003e\n            \u003ctd\u003eCompliance\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eVisible Particle\u003c\/td\u003e\n            \u003ctd\u003eShould be no visible particle\u003c\/td\u003e\n            \u003ctd\u003eCompliance\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eMoisture\u003c\/td\u003e\n            \u003ctd\u003e≤4.0%\u003c\/td\u003e\n            \u003ctd\u003e1.0%\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003epH\u003c\/td\u003e\n            \u003ctd\u003e6.5~7.5\u003c\/td\u003e\n            \u003ctd\u003e7.2\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003ePurity\u003c\/td\u003e\n            \u003ctd\u003e\u0026gt;90.0%\u003c\/td\u003e\n            \u003ctd\u003eCompliance\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eEndotoxin\u003c\/td\u003e\n            \u003ctd\u003e\u0026lt;0.1EU\/μg\u003c\/td\u003e\n            \u003ctd\u003e\u0026lt;0.1EU\/μg\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eMycoplasma\u003c\/td\u003e\n            \u003ctd\u003eShould be negative\u003c\/td\u003e\n            \u003ctd\u003eNegative\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eSterility\u003c\/td\u003e\n            \u003ctd\u003eNo growth\u003c\/td\u003e\n            \u003ctd\u003eNo growth\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eBiological Activity\u003c\/td\u003e\n            \u003ctd\u003eCell proliferation assay was performed with TF-1 cells, and the activity ≥ 1×10\u003csup\u003e6\u003c\/sup\u003e IU\/mg\u003c\/td\u003e\n            \u003ctd\u003e7.9×10\u003csup\u003e6\u003c\/sup\u003e IU\/mg\u003c\/td\u003e\n          \u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Features --\u003e\n  \u003cdiv class=\"biofargo-section\"\u003e\n    \u003ch2 class=\"biofargo-h2\"\u003eFeatures\u003c\/h2\u003e\n    \u003cul class=\"biofargo-checklist\"\u003e\n      \u003cli\u003e\n        \u003cspan class=\"biofargo-check\"\u003e✔\u003c\/span\u003e\n        \u003cstrong\u003eHigh Biological Potency (TF-1 Validated)\u003c\/strong\u003e\n        \u003cp\u003eDemonstrates strong proliferative activity in TF-1 cell assays (7.9 × 10\u003csup\u003e6\u003c\/sup\u003e IU\/mg), ensuring reliable Th2-skewing and DC induction performance.\u003c\/p\u003e\n      \u003c\/li\u003e\n      \u003cli\u003e\n        \u003cspan class=\"biofargo-check\"\u003e✔\u003c\/span\u003e\n        \u003cstrong\u003eHEK293 Expression with Authentic Glycosylation\u003c\/strong\u003e\n        \u003cp\u003eMammalian expression preserves native folding and glycosylation, supporting consistent IL-4Rα\/γc signaling activation.\u003c\/p\u003e\n      \u003c\/li\u003e\n      \u003cli\u003e\n        \u003cspan class=\"biofargo-check\"\u003e✔\u003c\/span\u003e\n        \u003cstrong\u003eLow Endotoxin, Cell-Therapy Compatible\u003c\/strong\u003e\n        \u003cp\u003eEndotoxin level \u0026lt; 0.1 EU\/µg and mycoplasma-negative, suitable for immune cell culture and CGT research workflows.\u003c\/p\u003e\n      \u003c\/li\u003e\n      \u003cli\u003e\n        \u003cspan class=\"biofargo-check\"\u003e✔\u003c\/span\u003e\n        \u003cstrong\u003eBatch-to-Batch Consistency\u003c\/strong\u003e\n        \u003cp\u003eManufactured under controlled QC standards with verified purity (\u0026gt;90% SDS-PAGE), minimizing experimental variability.\u003c\/p\u003e\n      \u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Applications --\u003e\n  \u003cdiv class=\"biofargo-section\"\u003e\n    \u003ch2 class=\"biofargo-h2\"\u003eApplications\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-grid\"\u003e\n      \u003cdiv class=\"biofargo-app-card\"\u003e\n        \u003ch4\u003eMonocyte-Derived Dendritic Cell (imDC) Generation\u003c\/h4\u003e\n        \u003cp\u003eUsed synergistically with GM-CSF to suppress CD14 expression and drive standardized immature DC differentiation.\u003c\/p\u003e\n      \u003c\/div\u003e\n      \u003cdiv class=\"biofargo-app-card\"\u003e\n        \u003ch4\u003eT-Cell Polarization Studies\u003c\/h4\u003e\n        \u003cp\u003eDirects naïve CD4⁺ T cells toward Th2 lineage via STAT6 activation, supporting immune-profiling research.\u003c\/p\u003e\n      \u003c\/div\u003e\n      \u003cdiv class=\"biofargo-app-card\"\u003e\n        \u003ch4\u003eCAR-T \/ CGT Process Development\u003c\/h4\u003e\n        \u003cp\u003eModulates Th1\/Th2 balance during T-cell manufacturing to optimize functional phenotype for solid tumor research.\u003c\/p\u003e\n      \u003c\/div\u003e\n      \u003cdiv class=\"biofargo-app-card\"\u003e\n        \u003ch4\u003eMast Cell \u0026amp; Allergy Research Models\u003c\/h4\u003e\n        \u003cp\u003eProlongs mast cell survival in vitro, enabling stable allergen-response and IgE-related studies.\u003c\/p\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Stability \u0026 Storage --\u003e\n  \u003cdiv class=\"biofargo-section\"\u003e\n    \u003ch2 class=\"biofargo-h2\"\u003eStability \u0026amp; Storage\u003c\/h2\u003e\n    \u003cul style=\"padding-left:20px; margin:10px 0 0 0;\"\u003e\n      \u003cli style=\"margin-bottom:8px;\"\u003eLyophilized powder: Store at −20 °C in the original sealed container.\u003c\/li\u003e\n      \u003cli style=\"margin-bottom:8px;\"\u003eReconstitution: Reconstitute with sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4) prior to use.\u003c\/li\u003e\n      \u003cli style=\"margin-bottom:0;\"\u003eHandling: Aliquot after reconstitution and avoid repeated freeze–thaw cycles.\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Cautions (Universal) --\u003e\n  \u003cdiv class=\"biofargo-section\"\u003e\n    \u003ch2 class=\"biofargo-h2\"\u003eCautions\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-caution\"\u003e\n      \u003cp\u003e\u003cstrong\u003e• Research Use Only:\u003c\/strong\u003e This product is not intended for diagnostic or therapeutic use.\u003c\/p\u003e\n      \u003cp\u003e\u003cstrong\u003e• Handling:\u003c\/strong\u003e Prepare solutions using sterile technique. Avoid microbial contamination to maintain activity.\u003c\/p\u003e\n      \u003cp\u003e\u003cstrong\u003e• Storage:\u003c\/strong\u003e Follow recommended storage conditions. Repeated freeze–thaw cycles may reduce performance.\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Documents --\u003e\n  \u003cdiv class=\"biofargo-section\"\u003e\n    \u003ch2 class=\"biofargo-h2\"\u003eDocuments\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-docs\"\u003e\n      \u003ca class=\"biofargo-btn primary\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Data_Sheet-Recombinant_Human_IL-4_Protein.pdf?v=1772440867\" target=\"_blank\" rel=\"noopener\"\u003e📄 TL-301 Data Sheet\u003c\/a\u003e\n      \u003ca class=\"biofargo-btn outline\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS-Recombinant_Human_IL-4_Protein.pdf?v=1772440868\" target=\"_blank\" rel=\"noopener\"\u003e📄 TL-301 SDS\u003c\/a\u003e\n      \u003ca class=\"biofargo-btn outline\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA-Pr2406006-TL-301-0100.pdf?v=1772440867\" target=\"_blank\" rel=\"noopener\"\u003e📄 TL-301 COA\u003c\/a\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"T\u0026L","offers":[{"title":"100μg","offer_id":51379496222901,"sku":"TL-301-0100","price":400.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG-TL-301-0100.png?v=1772441558","url":"https:\/\/biofargo.com\/products\/recombinant-human-il-4-protein","provider":"Biofargo","version":"1.0","type":"link"}