Description

IL-3 (Interleukin-3), also known as Multi-CSF, is a cytokine secreted by activated T cells, mast cells, and natural killer (NK) cells. It exerts its function by binding to the IL-3 receptor complex, composed of IL-3Rα (CD123) and the common beta chain (CD131), thereby activating downstream signaling pathways such as JAK/STAT, MAPK, and PI3K/Akt. IL-3 plays a critical regulatory role in both hematopoietic and non-hematopoietic systems. It promotes the survival and proliferation of hematopoietic stem and progenitor cells, and supports the growth of myeloid cells, including granulocytes, monocytes, and mast cells. Furthermore, IL-3 enhances the antigen-presenting capacity of dendritic cells (DCs) and stimulates B cell proliferation and antibody secretion.

Specifications

Product Details
Cat. No.	Y02121H
Purity	≥95%, by SDS-PAGE (under conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.02-0.1 ng/mL.
Accession	P08700
Species	Human
Expression System	E. coli
Mol. Weight	15.1 kDa
Expression Region	Ala20-Phe152
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm filtered solution containing 10 mM PB, 5% Trehalose and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Key hematopoietic growth factor promoting proliferation of multipotent progenitors.

Immunology

Supports myeloid immune cell generation and function.

Stem Cell Research

Widely used for hematopoietic stem/progenitor cell expansion in vitro.

Cell Biology

Controls survival and differentiation via STAT and PI3K pathways.

Data Figures

Figure 1: Measured in a cell proliferation assay using TF-1 cells.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human IL-3