Description

IL-33 (Interleukin-33) is a cytokine belonging to the IL-1 family and is mainly expressed by epithelial cells, endothelial cells, and fibroblasts. It signals through the ST2 (IL-1RL1) receptor in complex with IL-1RAcP, activating downstream pathways such as NF-κB and MAPK to initiate type 2 immune responses. Functionally, IL-33 acts as an “alarmin” released upon tissue damage, promoting activation of Th2 cells, ILC2s, mast cells, and eosinophils, and inducing the production of IL-5 and IL-13. In *in vitro* cell and organoid models, IL-33 is commonly used to simulate tissue injury–associated inflammation and type 2 immune microenvironments, particularly in studies of allergy, asthma, and epithelial–immune interactions.

Specifications

Product Details
Cat. No.	Y12821H
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤100 EU/mg by the LAL method.
Bioactivity	Measured by its ability to induce IL-8 secretion by HUVEC human umbilic vein endothelial cells. The ED50 for this effect is ≤1.0 ng/mL.
Accession	NP_001186569.1
Species	Human
Expression System	E. coli
Expression Region	Ser112-Thr270
Tag Info	Tag free
Form	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at-20°C to-80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Influences activation of myeloid and lymphoid immune cells.

Immunology

Acts as an “alarmin” initiating type 2 immune responses.

Cell Biology

Mediates stress and tissue damage signaling.

Data Figures

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human IL-33