Description

The recombinant human IL-21-Fc fusion protein is an innovative biologics engineered by covalently linking the human IL-21 pleiotropic cytokine with an immunoglobulin Fc domain through genetic recombination technology.

As a γc-chain cytokine family member secreted by antigen-activated CD4+ T cells (including Tfh and Th17 subsets) and NKT cells, IL-21 activates the JAK1/JAK3-STAT1/STAT3 signaling cascade via its heterodimeric receptor complex (IL-21R/γc), orchestrating immune cell functions: potentiating CD8+ T/NK cell-mediated tumor cytotoxicity, promoting Th17 polarization, suppressing Treg differentiation, and driving antibody affinity maturation through coordinated Tfh-B cell interactions.

The Fc fusion design not only extends serum half-life but also enhances immunomodulatory efficacy via Fcγ receptor-mediated targeted delivery, demonstrating unique therapeutic potential in cancer immunotherapy and autoimmune disease intervention.

In cell therapy manufacturing, the recombinant human IL-21-Fc fusion protein serves as a critical culture supplement to enhance the proliferation efficiency and effector cytotoxicity of engineered T/NK cells via JAK-STAT pathway activation, while promoting memory T-cell phenotypes and suppressing activation-induced cell exhaustion (AICD), thereby supporting the generation of highly potent and durable antitumor immune cell products.

Specifications

Cytokines Details
Synonyms	IL21, Za11, Interleukin-21
Accession	UniProt Q9HBE4-1
Amino Acid Sequence	Gln29–Ser126
Fusion Tag	Human IgG1 Fc fragment fused to the C-terminus
Expression System	CHO cells
Predicted Molecular Weight	41.9 kDa
Purity	> 95% as determined by SDS-PAGE and HPLC
Endotoxin	< 0.01 EU per 1 μg of protein (LAL method)
Biological Activity	Induces IFN-γ secretion in human NK-92 lymphoma cells with an ED₅₀ ≤ 50 ng/mL
Formulation	Lyophilized from sterile 20 mM phosphate-buffered saline (PBS), pH 7.4, typically containing 6–8% (w/v) mannitol as a stabilizing protectant

Features

✔ Fc-Fusion Enhanced Durability
Human IgG1 Fc fusion extends functional persistence and improves cytokine stability.
✔ Potent JAK-STAT Activation
Activates IL-21R/γc–JAK1/JAK3–STAT1/STAT3 signaling for strong immune modulation.
✔ High Biological Activity
Demonstrates robust IFN-γ induction in NK-92 cells with low ED₅₀ performance.
✔ DMF Filed (039117)
Supports regulatory readiness for GMP cell and gene therapy workflows.

Applications

T Cell Expansion and Effector Enhancement

Promotes proliferation, cytotoxic function, and memory phenotype in engineered T cells.

NK Cell Cytotoxicity Optimization

Enhances NK cell antitumor activity while limiting activation-induced exhaustion.

Cell Therapy Manufacturing

Ideal cytokine supplement for generating potent and durable T/NK cell products.

Cancer Immunotherapy Research

Supports studies focused on immune activation, memory formation, and antitumor efficacy.

Stability & Storage

√ Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored inthe original sealed container under desiccant.
√ Reconstitution: Dissolve in sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4)maintaining final concentration ≥100 µg/mL to prevent adsorption.
√ Handling: Aliquot to avoid repeated freeze-thaw cycles.

Cautions

• Research Use Only: This product is not intended for diagnostic or therapeutic use.

• Handling: Follow recommended storage and handling instructions to maintain product stability.

• Support: Contact technical support if assistance is required.

Documents

📄 GMP-TL509 Data Sheet 📄 GMP-TL509 SDS 📄 GMP-TL509-0050 COA 📄 GMP-TL509-0100 COA

Recombinant Human IL-21 Protein