Description

Activated T cells generally cannot survive for a long time in vitro culture, and the addition of IL-2 can promote their long-term and sustained proliferation.

The surface of stationary T cells does not express IL-2R and does not respond to IL-2. T cells activated by mitogen or other stimuli can express IL-2R and become target cells of IL-2. IL-2 can also induce increased IL-2R expression in target cells.

The expression of IL-2R on T cells is transient, generally reaching its peak 2–3 days after activation and disappearing around 6–10 days. With the disappearance of IL-2R, T cells lose their ability to respond to IL-2.

Therefore, in order to maintain the long-term growth of normal T cells in vitro, it is necessary to constantly stimulate T cells with mitogen or other stimulators to maintain the expression of IL-2R.

NK cells are the only lymphoid cells that normally express IL-2R and therefore always maintain reactivity to IL-2. However, only the IL-2R β chain and γ chains are expressed on stationary NK cells, which have a low affinity for IL-2 and can only react with high concentrations of IL-2.

Once NK cells are activated, they express the IL-2R α chain, becoming a high-affinity receptor.

Specifications

Cytokines Details
Expression Host	E. coli
Synonyms	TCGF, Lymphokine, Interleukin
Purity	> 90% as determined by SDS-PAGE
Biological Activity	Determined by the dose-dependent stimulation of CTLL-2 cell proliferation, corresponding to an activity of ≥ 1 × 10⁷ U/mg
Endotoxin	< 0.1 EU per 1 μg of protein, determined by the LAL method
Molecular Mass	The recombinant human IL-2 protein has a predicted molecular mass of 15.4 kDa
Formulation	Lyophilized from sterile PBS, pH 7.4. Typically supplemented with 6% mannitol as a stabilizing protectant prior to lyophilization

Features

✔ High-Potency IL-2: ≥1 × 10⁷ U/mg bioactivity ensures strong and reproducible proliferation of activated T and NK cells.
✔ Tag-Free Design: Eliminates Fc-mediated off-target effects, supporting clean signaling in immune cell culture.
✔ Ultra-Low Endotoxin: <0.1 EU/µg endotoxin, suitable for sensitive cell cultures and therapy process development.
✔ Optimized Growth: Effectively sustains proliferation during the IL-2R expression window following activation.
✔ Stable Formulation: Mannitol-protected lyophilized format ensures excellent stability and reliable performance.

Applications

CAR-T/TCR-T Expansion

Supports controlled and scalable expansion of genetically modified T cells during ex vivo manufacturing.

NK Cell Activation

Promotes NK cell proliferation and enhances cytotoxic activity and cytokine secretion.

TIL & CIK Culture

Enables sustained growth of tumor-reactive and cytokine-induced killer cells in vitro.

Signaling Studies

Used to study IL-2/IL-2R signaling, receptor dynamics, and lymphocyte proliferation mechanisms.

Data Figures

Figure 1: Recombinant Human IL-2 Protein Analysis

Stability & Storage

Lyophilized preparation can be stored at 2-8 ℃.
6 months at -20℃ under sterile conditions after reconstitution.
12 months at -80℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.
Avoid repeated freeze-thaw cycles.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 GMP-TL906 Data Sheet 📄 GMP-TL906 SDS 📄 GMP-TL906 COA

Recombinant Human IL-2 Protein (High-Efficiency)